In order to reveal the influence of slope aspects on soil ciliates community, the soil ciliates communities and environmental factors were investigated in subalpine meadow of Gannan in April and July of 2015. The results showed that 97 species of ciliates were identified in spring, belonging to 9 classes, 17 orders, 32 families and 50 genera, and 141 species of soil ciliates were identified in summer, belonging to 9 classes, 18 orders, 32 families and 55 genera. Soil ciliate community had obvious surface aggregation. Soil ciliates density and taxa were significantly lower in spring than in summer, and the density of soil ciliates was the highest on the south slope in spring and on the west slope in summer. Soil water content and nutrient content were higher, and pH and soil temperature were lower on the north slope, while the situation was opposite on the south slope, and the environmental factors were moderate on the west slope. RDA analysis showed that the response of soil ciliates was different to environmental factors in subalpine meadows of Gannan. Most of taxa were suitable for the moderate habitat conditions of water, heat and nutrient on the west slope. Nassulidae and Cyrtolophosidae adapted to the condition of rich soil total phosphorus on the southwest slope, while Cohnilemebidae adapted to the condition of high soil water content and rich nutrients on the north slope.