cje
Previous Articles Next Articles
LI Guang-wei*, CHEN Xiu-lin, SHANG Tian-cui
Online:
Published:
Abstract: To explore the OBP genes of antennae Anoplophora nobilis Ganglbauer, the transcriptome of female antennae was generated using Illumina HiSeq2500, the OBPs were identified by GO annotation and traditional DNA sequencing, and the tissue distribution of OBPs was performed by semiquantitative PCR. The results showed that 16 OBPs were identified from female antennae A. nobilis, including 11 Minus-C OBPs, three Classical OBPs and two Plus-C OBPs. Each OBP with a signal peptide at Nterminal consisted of 16-25 amino acids. OBPs in each subfamily have their own typical conservative Cys residues. The AnobOBPs are highly divergent and the sequence similarities range from 8.00% to 48.44%. Phylogenetic analysis showed that although OBPs of the same or different species are highly divergent, the great majority of OBPs in each subfamily is still clustered into independent clade, suggesting that they have a common ancestor. The expression levels of 16 female antennae AnobOBPs were significantly different, with the RPKM values ranging from 12.0 to 9065.5. AnobOBP1, AnobOBP2, AnobOBP3 and AnobOBP7 exclusively expressed in antennae, and the other AnobOBPs expressed in antennae and other tissues, including head, thorax, abdomen, leg and wings, indicating that the OBPs in insects may also participate in other functions in addition to chemosensation. This study established a foundation for determining the chemoreception molecular mechanism of A. nobilis, which would provide a new perspective for controlling the borer pests by using semiochemicals.
LI Guang-wei*, CHEN Xiu-lin, SHANG Tian-cui. Identification and tissue distribution of odorant binding proteins of female antennae Anoplophora nobilis Ganglbauer (Coleoptera: Cerambycidae).[J]. cje.
0 / / Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.cje.net.cn/EN/abstract/abstract22767.shtml
https://www.cje.net.cn/EN/Y2017/V36/I6/1678