Abstract: Some Sb(Ⅲ)tolerant plant growth-promoting bacteria were isolated from the heavy metals contaminated soil around the Xikuangshan antimony mine, Hunan Province, by the CDM medium. Based on morphological observation, physiologicalbiochemical test and 16S rRNA gene sequence analysis, these bacteria were identified. Their biological characteristics including Sb(Ⅲ) tolerance, absorption and oxidation, growthpromoting properties, adaptabilities to pH and salinity, and their growthpromoting effects on rape seedlings were investigated by a pot experiment. The results showed that four Sb(Ⅲ)tolerant and growthpromotion bacteria were isolated (XKS-1, XKS-2, NXH-2, NXH-3) belonging to the genera of Pseudomonas, Ensifer, Raoultella and Stenotrophomonas. Their minimum inhibitory concentrations (MIC) of Sb(Ⅲ) were higher than 25 mmol·L^-1, and they could adsorb and oxidize Sb(Ⅲ). These four strains could produce indoleacetic acid (IAA) and siderophore. The effects of pH and salinity on the growth of four bacteria were different. Compared with the noninoculated control, height and root length, chlorophyll a and chlorophyll b contents of rape seedlings treated with four bacteria were increased by 4%-26%, 13%-32%, 23%-35% and 5%-32% respectively at low and intermediate Sb concentrations. These four bacteria had no significant effect at high Sb concentration. Results from this study highlight that these four Sb(Ⅲ)tolerant bacteria had strong adaptability to the environment, which could be used for bioremediation of soil polluted by antimony.