Abstract: Rhizosphere micro-environment is the bridge of communication between plant and soil, and the priority area that responds to the environmental stress. Changes in root architecture, type and quantity of exudates may impact rhizosphere microorganisms and soil fauna structure and diversity, as the roots are main regulators of rhizosphere. However, there are complicated interactions between plant roots, microorganisms and soil animals in the rhizosphere. Soil animals’ disturbance and microbial decomposition can improve rhizosphere soil properties, and enhance plant stress and nutrient use efficiency, thereby promoting the growth of plant roots. Based on the studies of the physical and chemical disturbances of external microenvironment of root rhizosphere, interactive disturbance of roots and microbes of internal rhizosphere, physical disturbance of roots and soil animals, chemical disturbances with root exudates as the medium, we clarified the interaction mechanisms of disturbance in internal and external environment of rhizosphere, and made prospects for further research in this field.