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Isolation and identification of ammonifying bacteria in the flue-cured tobacco and characteristics of degrading organic nitrogen.

ZHOU Jia-xi1, ZHANG Xiao-min2, HU Da-ming2, HUI Jian-quan2, YU Tao1, ZOU Xiao1*#br#   

  1. (1 Institute of Fungal Resource, College of Life Sciences, Guizhou University, Guiyang 550025, China; 2 Guizhou Tobacco Industry Limited Liability Company, Guiyang 550025, China).
  • Online:2016-11-10 Published:2016-11-10

Abstract: During a survey of ammonifying bacteria, three highactivity strains of ammonifying bacteria, viz., GZUIFR-YC01, GZUIFR-YC02, and GZUIFR-YC03, were screened from flue-cured tobacco (Hunan A3B1 and Yunnan C3F produced in 2013) after enrichment culturing. Based on the morphological, physiological and biochemical characteristics and phylogenetic analysis, GZUIFR-YC01, GZUIFR-YC02, and GZUIFR-YC03 were identified as Stenotrophomonas maltophilia, Lysinibacillus fusiformis, and Brevibacillus parabrevis, respectively. In addition, L. fusiformis (GZUIFR-YC02) grew better than S. maltophilia (GZUIFR-YC01) and B. parabrevis (GZUIFR-YC03). While each of the three strains had a strong ability to degrade organic nitrogen, L. fusiformis (GZUIFR-YC02) was the best. This study provides reference to nitrogen transformation by microbes during the progress of tobacco leaf aging.

Key words: vegetable residue, solar greenhouse, vegetable yield., compost, root environment