From August to September in 2010 and March to September in 2011, 36 infrared cameras were installed for monitoring the effect of tourist roads on mammal activities in Shennongjia National Nature Reserve of Hubei Province. In this study, 432 independent photos were collected and 20 mammal species were identified. The identified species belonged to 12 families and 5 orders. At distances of 0-300 and 300-600 m from the tourist roads, the frequencies of mammal activity captured by infrared cameras changed significantly, increasing from 17.25% to 26.89%, showing that the mammals had certain avoidance to the tourist roads. The daily activity and distribution zones of four ungulate species showed different responses to the tourist roads. The diurnal activities of the tufted deer (Elaphodus cephalophus) and the goral (Naemorhedus goral) changed significantly with their proximity to the tourist roads; however, the tourist roads had no effect on sika deer (Cervus nippon) or wild boar (Sus scrofa). We suggest that additional comprehensive surveys and monitoring are necessary to better understand the effect of tourist roads on wildlife.