The characteristics of rubber chilling injury were analyzed by annual integrative chilling injury indexes based on the “rubber chilling injury grade (QX/T 169-2012)” of China Meteorological Administration (CMA) and the related meteorological data during 1961-2010 in major rubber producing areas in China. The results indicated that rubber radiation chilling injury and advection chilling injury showed obvious spatial differences. The high annual average accumulative temperatures of radiation type rubber chilling injury appeared in Yunnan and Fujian provinces, while the high annual average accumulative temperatures of advection type rubber chilling injury appeared in Guangxi, Fujian, Guangdong provinces and Pingbian of Yunnan Province. The occurrence frequencies of moderate, severe, severe rubber chilling injury showed a decreasing tendency, while light rubber chilling injury showed an increasing tendency during 1961-2010. The average occurrence frequencies of light, medium, heavy and severe rubber chilling injury were 3.50%, 28.24%, 14.93%, and 15.93%, respectively. In Jinghong and Mengna of Yunnan Province and Hainan Island, the occurrence frequencies of rubber chilling injury were far lower than those in the whole study area. The results provide scientific reference for decision-making in coping with the rubber chilling injury in China.