At three simulated acid rainfall intensities with pH values of 2.5 (severe), 4.0 (medium) and 5.6 (light), the responses of chlorophyll fluorescence parameters of Myrica rubra seedlings were studied in three stress ways, i.e. only the aboveground part of seedlings exposed to acid rain (T1), both of the seedlings and soil exposed to acid rain (T2), only the soil exposed to acid rain (T3) compared with blank control (CK). The relative chlorophyll content (SPAD), PS Ⅱ maximal photochemical efficiency (F_v/F_m), PS Ⅱ potential activity (F_v/F_o), and PS actual photochemical quantum yield (Yield) under each stress showed T3>T1>T2 within six months, and T1>T3>T2 after six months. It indicated that T2 had the strongest inhibition on seeding. T1 had the stronger inhibition at the early stage while T3 showed the stronger inhibition at the later stage. All chlorophyll fluorescence parameters in the three stress ways were lower than those in the CK group under the severe acid rainfall intensity, while T1 and T3 had higher values than the CK group under the medium and light acid rainfall intensities. It indicated that M. rubra seedlings had strong tolerance ability to acid rain. Acid rainfall at certain intensities could increase the values of chlorophyll fluorescence parameters of M. rubra. The values of F_v/F_m, F_v/F_o and Yield were higher in summer and autumn than in winter and spring. Significant differences in the values of F_v/F_m and F_v/F_o were found among T1, T2 and T3.