Abstract: The effects of the interaction between Suillus grevillei and Trichoderma virens on Pinus sylvestris var. mongolica were studied using plant physiology and biochemistry. Seedling growth and physiological parameters were determined, including the biomass, photosynthetic pigment content, soluble protein content, antioxidant enzyme activities, rhizosphere soil enzyme activities and protective enzyme activities. In addition, an optimal resistance system involving T. virens, mycorrhizal fungus (Suillus grevillei) and P. sylvestris var. mongolica seedlings was constructed. Synergies between S. luteus and T. virens were observed and most of the parameters of Pinus sylvestris var. mongolica seedlings inoculated with S. luteus 30 days + T. virens were higher than other treatments. After three months, when compared the control, the S. luteus 30 days + T. virens treatment gave increases in height (43%), collar diameter (55.6%), soluble protein content (176.9%), chlorophyll a (76.2%), chlorophyll b (64.1%), carotenoids (103.6%), CAT activity (45.5%), SOD activity (43.7%), β-1,3glucanase activity (28%), chitinase activity (40%), rhizosphere soil urease activity (120%), and phosphatase activity (100%). These results indicated that there may be a stimulating factor between S. grevillei and T. virens when they are inoculated together (S. grevillei 30 days + T. virens).

Key words: river microhabitat, macroinvertebrate, heterogeneity, geostatistics