Abstract: Cadmium is a non-essential element for plant growth, however, it could be absorbed and remained easily, and make an obvious damage to tobacco (Nicotiana tabacum L.). In this study, tobacco leaves were induced to produce callus by the minimal medium (MS + 1.0 mg·L^-1 6BA + 0.5 mg·L^-1 NAA). After 45 days, with a large amount of callus, the activities of related enzymes (SOD, CAT, APX, MDAR, GR and DHAR) in the antioxidant system, the levels of reactive oxygen species (O^-·₂, H₂O₂), and malondialdehyde (MDA) content were measured every three days. The results showed that the reactive oxygen species enhanced, and the activities of antioxidantive enzymes increased in tobacco callus under the cadmium stress within 45-51 days. However, after 51-57 days the activities of antioxidantive system, and membrane lipid peroxidation were aggravated, indicating that the increasing activities of antioxidantive system could not resist the longterm (54-57 days) high cadmium stress.

Key words: electroantennograph, Chilo suppressalis, temperature, sex pheromone communication