Abstract: Ten species of low temperature-tolerant aquatic macrophytes were chosen to construct four combinations of aquatic macrophytes to study their purification efficiency on eutrophic water body in winter. In non-macrophyte treatment (CK), the removal efficiency of water total nitrogen (TN), nitrite nitrogen (NO_x-N), ammonium nitrogen (NH₄-N), and total phosphorus (TP) was 40.0%, 25.9%, 27.3%, and 64.5%, respectively. Combination 1 (Iris hexagonus Hybrid + Rumex japonicas + Acorus gramineus “Ogan” + Juncus inflexus “Blue Arrows”) grew luxuriantly in the test eutrophic water body, and performed best in decreasing water TN, NO_x-N, NH₄-N, and TP, with the removal efficiency being 47.8%, 52.2%, 32.4%, and 70.1%, respectively. Combination 2 (I. hexagonus Hybrid + R. japonicas + A. gramineus “Ogan” + Hemerocallis middendorfii) could survive and had definite growth amount in the test eutrophic water, and the removal efficiency of water TN, NO_x-N, NH₄-N, and TP was 44.2%, 58.5%, 34.6% and 67.8%, respectively. In contrast, both the combination 3 (R. japonicas + Lolium perenne + Chlorophytum comosum + Juncus pallidus R. Br. “Javelin”) and the combination 4 (R. japonicas + L. perenne + C. comosum + Carex tristachya) only had an obvious efficiency in removing NOx-N but less efficiency in removing TN, NH₄-N, and TP, due to the poor growth of C. comosum and C. tristachya. It was suggested that combinations 1 and 2 had better purification efficiency on eutrophic water body, being the suitable combination forms of aquatic macrophytes floatingbed under low temperature in winter.