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cje ›› 2011, Vol. 30 ›› Issue (02): 315-319.

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Screening of PAHs-degrading bacterial strains and determination of their capability in degrading PAHs.

YUAN Shuang1, LIANG Cheng-hua1**, LI Feng-mei2,3, GUO Shu-hai2   

  1. 1Shenyang Agricultural University, Shenyang 110866, China|2Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China|3Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Online:2011-02-08 Published:2011-02-08

Abstract: Eight PAHs-degrading bacterial strains were screened out from the enriched culture with PAHs-contaminated soils in Benxi City of Liaoning Province, Northeast China, and the capability of the strains in degrading phenanthrene, phyrene, and benzo[a]pyrene with an initial concentration of 50, 50, and 5 mg·L-1 in medium was determined after 28-day incubation at 28 ℃. Among the strains screened out, B3 showed the best degrading effect, with the degradation rate of phenanthrene, phyrene, and benzo[a]pyrene being 88.4%, 54.0%, and 68.4%, while the degradation rate of these compounds by the mixed 8 strains was 87.7%, 35.3%, and 42.0%, respectively. The strain B3 was identified as Pseudomonas sp., based on its physiological and biochemical characteristics and the sequence analysis of 16S rRNA.

Key words: Intertidal zone, Community diversity, Community, Changshan archipelago