Abstract: The body size and reproductive life history of three gecko (Gekko ja ponicus) populations in Zhejiang Province of China were investigated, and the gecko eggs were incubated at 24 ℃, 28 ℃, and 31 ℃ to study the phenotypic tra its of the resultant hatchlings. Adult G. japonicus showed significant diffe rences in body sizes among the populations and between the sexes. The adults fro m Quzhou and Wenzhou populations were larger in snout-vent length and body mass than those from Hangzhou population, and the females in all the three populatio ns were bigger than the males. Egg mass and clutch mass did not differ among the three populations, whereas relative clutch mass was larger for Hangzhou populat ion than for Quzhou and Wenzhou populations. Incubation temperature affected the hatch duration and the tail length and head width of hatchlings significantly, but less affected the snout-vent length, body mass and locomotor performance of the hatchlings. Such effects of incubation temperature on eggs and hatchlings d id not show different patterns among the populations. This study demonstrated th e micro-geographic variations in body size, reproductive output, and hatching t raits of G. japonicus.

Key words: Marine bacterium (Pseudomonas sp), Culture condition, Fatty acids