Abstract: The metallothionein (MT) was produced with cucumber leaves under treatment of cadmium. After purification, the MT was coupled with BSA, and then injected subcutaneously or intracutaneously into the back and auricular vein of rabbit to generate antibodies against MT. The measurement showed that the ratio of antibody was 1 to 16. IgG was isolated and purified by (NH₄)₂SO₄saturated precipitation and Sephadex G-200 column. The content of IgG was up to 0.9364 mg·ml^-1 through UV determination. Dot-immunobinding assay revealed that the minimal level of antigen detected by Cd-MT antibody was 200 pg·ml^-1.

Key words: Leaching rate, Controlled-release N fertilizer, Ammonia volatilization, Urease activity, pH value