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Molecular ecology of anammox bacteria in constructed rapid infiltration system.

JIANG Xin1,2;MA Ming-chao1;LI Jun2;ZHONG Zuo-shen1   

  1. 1School of Water Resources and Environment, China University of Geoscie
    nces, Beijing 100083, China;2Institute of Agricultural Resources and Regi
    onal Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2007-07-20 Revised:1900-01-01 Online:2008-04-10 Published:2008-04-10

Abstract: In this paper, the specific16S rDNA primers of anammox bacteria from constructed rapid infiltration system (CRI) were used for PCR amplification, cloning, and sequencing, and the phylogenetic tree was constructed. The anammox bacteria were found at the depth of 50 cm in CRI, and the 16S rDNA partial sequence was 831 bp. Phylogenetic analysis indicated that the anammox bacteria were closed to anoxic biofilm clone Pla1-48, uncultured anoxic sludge bacterium KU2, and anaerobic ammonium-oxidizing planctomycete KOLL2a, with the similarity of DNA sequence being above 97%. The finding of anammox bacteria in CRI provided a new approach for the technical improvement and the increase of denitrification in CRI.

Key words: Black soil, Farmyard manure, Microbial biomass, Growth season, Dynamic change