Abstract: By using microsatellite technology and 17 pairs of microsatellite DNA primers, the genetic diversities of cultured and wild Cyprinus carpio populations in Xiangjiang River were studied. 15 of the 17 pairs of microsatellite primers were amplified with clear bands, among which, 13 pairs presented polymorphism. 2-12 alleles per pair in the two populations were amplified, with 35 alleles coexisted in both of the two populations and 55 alleles in each population. The average number of the alleles per pair was 6.92, and the frequency of the alleles was 0.0667-0.8333. The average heterozygosity and polymorphism information contents of cultured and wild C. carpio populations were 0.5688 and 0.5152, and 0.5860 and 0.5347, and the genetic similarity index and genetic distance were 0.6762 and 0.3238, respectively, indicating that the genetic diversity of the two populations was relatively abundance, and the genetic variability was high.

Key words: Plastic film mulching, Soil microbial biomass carbon, Spring wheat, Plant nutrition, Soil quality, Semi-arid Loess Plateau