Abstract: Pseudostellaria heterophylla is a valuable herbal medicine, which experiences serious yield decline under consecutive monoculture. This study aimed to evaluate the effects of different microbial fertilizers using plant growthpromoting rhizobacteria (Bacillus subtilis andBurkholderia as main component) on the continuous cropping obstacle ofP. heterophylla, taking mixture of soybean pulp and pig manure as a control.P. heterophylla rhizosphere soils were collected from six treatments: basal control (CK), firstrotation planting (FP), secondrotation planting (SP),Bacillus subtilis fertilizer (No. 1),Burkholderia fertilizer (No. 2), Bacillus subtilis and Burkholderia mixed fertilizer (No. 3), to investigate physicochemical properties and fungal community structure inP. heterophylla rhizosphere soils. The results showed that three kinds of microbial fertilizers (No. 1, No. 2 and No. 3) significantly increased the yield of P. heterophylla by 107%, 112% and 96% compared to SP treatment, respectively. In addition, these bio-fertilizers significantly improved soil pH and available nitrogen (AN) content but reduced the contents of available potassium (AK) and phosphorus (AP). These results indicated that large amounts of functional bacteria existed in the bio-fertilizers, which could effectively promote soil nutrientavailability. The DGGE results demonstrated that the fungal community structure under the treatments of bio-fertilizer No. 2 and No. 3 was similar to that in firstrotation planting (FP), which was further confirmed by qPCR results. Our results indicated that the application of bio-fertilizers could improve the yield by influencing soil microbial community structure and function in thecontinuous cropping systems of P. heterophylla. These results exemplify the potential of bio-fertilizers in reducing the effects of replanting disease and in improving soil quality, providing clues onoptimizing cultivation techniques ofP. heterophylla under continuous cropping.

Key words: peanut, variety, root nodule, nitrogen fixation characteristic, yield