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Effect of exogenous hormones on germination and antioxidant enzyme activity of seeds of Cyclobalanopsis chungii.

LAI Hua-yan1, WANG You-feng1,2, WU Kai1, MA Xiang-qing1, WU Peng-fei1*#br#   

  1. (1College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2Inner Mongolia Land Surveying and Planning Institute, Hohhot 010010, China).
  • Online:2017-02-10 Published:2017-02-10

Abstract: To solve the problems of quick seed germinating and artificial seedling difficulty of Cyclobalanopsis chungii, a precious timber species, the healthy, mature and nongerminating seeds were selected as experimental material. The antioxidant enzyme system activity in radicle and co-tyledon of the seeds was determined in 3, 5, 7, 9 and 13 days after treating with five different concentrations (0, 10, 20, 30, 40 mmol·L-1) of two kinds of hormone (gibberellin acid and abscisic acid). The results showed that there were significant differences in germination rate and antioxidant enzyme activity among different hormone treatments. With the increase of the gibberellin acid concentration, the germination rate of seeds increased markedly, but there was no significant difference among the treatments. The abscisic acid with low concentration (10-20 mmol·L-1) promoted the germination rate significantly, but not with high concentration (30-40 mmol·L-1). With extension of the processing time of exogenous hormones, POD and SOD activities in the radicle and cotyledon showed a tendency of increase firstly then exhibited irregular changes. Under different concentrations of gibberellin acid, CAT activity in the radicle increased gradually with prolonging time, whereas CAT activity in the cotyledon demonstrated an inverted “N” pattern. Applying with 40 mmol·L-1 abscisic acid could solve the problem of quick germinating by inhibiting PPO activity in the radicle and cotyledon.