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Screening and identification of bacteria for degradation of pesticides carbofuran and chlorpyrifos.

WANG Xin**, LU Jia-liang, LIU Li, CUI Li, LIANG Ji-yan   

  1. (School of Science, Shenyang University of Technology, Shenyang 110870, China)
  • Online:2014-03-10 Published:2014-03-10

Abstract: Several strains of bacteria were applied in the degradation experiments of carbofuran and chlorpyrifos, and the preponderant bacteria were identified by comparison. The LLBK2 and LLBK10 showed the best degradation effect on the 100 mg·L-1 of carbofuran, with the degradation rates of 58.0% and 60.0% in 48 hours, and 82.8% and 83.8% in the 72 hours, respectively. The LLBD2 and LLBD4 showed the best degradation effect on the 100 mg·L-1 of chlorpyrifos, with the degradation rates of 70.6% and 61.5% in the 48 hours, and 92.3% and 91.4% in 72 hours, respectively. Through further microbiological identification, LLBK2, LLBK10, LLBD2 could be classified as Bacillus circus. The accessing numbers of their 16S rDNA gene sequences in the GenBank were JQ917438, HQ833023, and JX847612, respectively. LLBD4 could be categorized as Bacillus, which has the GenBank accessing number of JX010962.

Key words: potential habitat, habitat, bamboo forest, population, correlation analysis.