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Ectomycorrhiza formation on Pinus tabulaeformis through Rhizopogon luteolus infection. 

SHAO Dong-hua1, YANG Xi-ping2, ZHANG Xiao-dong1, BAI Shu-lan1**, ZHENG Rong1, WANG Ju-gang1   

  1. (1 Forestry College of Inner Mongolia Agricultural University, Hohhot, 010019, China; 2 Garden Bureau of Wuhai, Wuhai 016000, Inner Mongolia, China)
  • Online:2013-01-10 Published:2013-01-10

Abstract: Taking  60-day-old Pinus tabulaeformis seedlings as test objects, a pot culture experiment under laboratory condition was conducted to investigate whether the inoculation with Rhizopogon luteolus could infect P. tabulaeformis and form ectomycorrhiza as well as the effectiveness of this infection on the growth of P. tabulaeformis. After two months of inoculation, the ectomycorrhiza formed, and the mycorrhizal colonization rate was over 80%. In terms of branching characteristics, the binary ectomycorrhiza accounted for 80%, and the rod-shaped one shared 20%. The mantle was white in color, quite distinct, and 90 μm in thickness. The diameter of the ectomycorrhizal branch was 0.4-0.6 mm, and the length of the branch was 1.5-2.5 mm. The extraradical hyphae were white and villous, and many of them were twisted as rhizomorphae, with a length of 3.5-5.5 mm. The ectomycorrhiza had prominent promotion effect on the growth of P. tabulaeformis roots. The root-shoot ratio of the mycorrhizal seedlings was 2.7 times of the control. After the formation of
ectomycorrhiza, the phosphorus absorption by P. tabulaeformi from the environment increased significantly.

Key words: North Loess Plateau, Alfalfa (Medicago sativa), Artificial grassland, Community succession, Plant diversity, Forest-steppe ecotone