Abstract: To understand the molecular mechanism of cucumber’s heat tolerance or heat injury, heat-tolerant cultivar ‘Xishuangbanna’ and heat-sentitive culticar‘Erzaozi’ were selected as test materials, and their seedlings were heat treated (38 ℃) for 2 hours. The gene was isolated with cDNA-AFLP technique, and the gene expression was detected by RT-PCR technique. A specific fragment of heat response, chrr182 (cucumber heat response-related), was isolated from the cDNA of ‘Erzaozi’ under heat stress, and the results of blastx in GenBank of NCBI indicated that chrr182 was in homology with transcription-repair coupling factor gene. The expression of chrr182 was enhanced under the inducement at 38 ℃ for 2 hour. The heat tolerance or heat injure of cucumber seedlings could be related to the response of transcription-repair coupling factor gene under heat stress.

Key words: Salix psammophila, Shrub, Plant morphology, Flow structure, Wind-prevention effect