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Effects of salt and water stress on seed germination of halophytes Kalidium foliatum and Halostachys caspica

ZENG Youling; CAI Zhongzhen; MA Ji; ZHANG Fuchun; WANG Bo   

  1. Key Laboratory of Molecular Biology, College of Life Science and Technology, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Xinjiang University, Urumqi 830046, China

  • Received:2005-12-28 Revised:2006-05-30 Online:2006-09-10 Published:2006-09-10

Abstract: The study showed that after, the seed germination of two halophytes kalidium foliatum and Halostachys caspica treated with different concentration NaCl solutions and iso-osmotic PEG was inhibited, and iso-osmotic PEG had a greater inhibitory effect than iso-osmotic NaCl, suggesting that osmotic stress was the main factor affecting halophytes seed germination. The inhibitory effects of NaCl and water stress mainly reflected in lowering seed germination rate, delaying initial germination time, and extending germination duration. Both of the halophytes had strong salt tolerance. When NaCl concentration was from 0 to 300 mmol·L-1, the relative germination rate of H. caspica was over 95%, and that of K. foliatum was over 80%, indicating that H. caspica was more salt-tolerant than K. foliatum. For H. caspica, its fit value of salt-tolerance was 332.5 mmol·L-1, critical value was 540 mmol·L-1, and the maximum value was 749 mmol·L-1, while for K. foliatum, the corresponding values were 246.5 mmol·L-1, 391.8 mmol·L-1 and 537 mmol·L-1 respectively. Low concentration NaCl solution promoted shoot growth. The two halophytes treated by 100 mmol·L-1 NaCl had the maximum length of root and shoot, and the shoot grew well.

Key words: Lathyrus sativus, EMS, 60Coy-ray(unit.Krad), ODAP(β-N-Oxalyl-α,β-diamino propionic acid)