Analysis methods of phytochelatins

Abstract

Abstract: Phytochelatins is a kind of peptides rich in cysteine,which can bind the ion of heavy metals.Phytochelatins has a common structure: [γ-Glu-Cys ]_nGly (n=2～11),and it is one of the key factors of plant heavy metal tolerance mechanism.Its analysis methods can be applied to study plant heavy metal tolerance mechanism.The article introduced the basic theory of phytochelatins analysis,and analysis methods including Spectrophotometer,HPLC and CE,among which the HPLC was presented in detail.

Key words: Polytrichum juniperum, Mire, Clone, Innate annual marker, Age structure

CLC Number:

Q503

ZHAO Di, XIA Lijiang, ZHU Yongguan. Analysis methods of phytochelatins[J]. cje, 2005, (3): 330-334.

References

[1]高华,张玉秀,柴团耀.2001.植物络合素和植物络合素合成酶的研究[J].西北植物学报,21(4):779～790.
[2]Aher BA, Price NM, Morel FMM. 1994. Phytochelatin production by marine phytoplankton at low free metal ion concentrations: Laboratory studies and field data from Massachusetts Bay[J]. PNAS, 91:8433～8436.
[3]Ebbs S, Lau I, Ahner B, et al. 2002. Phytochelatin synthesis is not responsible for Cd tolerance in the Zn/Cd hyperaccumulator Thlaspi caerulescens (J. & C. Presl) [J]. Planta, 214:635～640.
[4]Hart JJ, Wrlch RM, Norvell WA, et al. 2002. Measurement of thiol-containing amino acids and phytochelatin (PC2) via capillary electrophoresis with laser-induced fluorescence detection[J].Electrophoresis, 23: 81 ～ 87.
[5]Hartley-Whitaker J, Ainsworth G, Vooijs R, et al.2001. Phytochelatins are involved in differential arsenate tolerance in Holcus lanatus [ J ].Plant Phys., 126:299～306.
[6]Hiroki K, Kyoko S, Takashi Y, et al. 1998. Separation of respective species of phytochelatins and their desglycyl peptides (class Ⅲ metallothioneins) and the precursors glutathione and g-glutamylcysteine with capillary zone electrophoresis [ J ]. J. Chromatography A, 803: 315 ～ 320.
[7]Newton GL, Dorian R, Fahey RC. 1981. Analysis o( ) biological thiols: Derivatization with monobromobimane by reverse-phase high-performance liquid chromatography [ J ]. Anal. Biochem.,114: 383 ～ 387.
[8]Schmoger MEV, Oven M, Grill E. 2000. Detoxification of arsenic by phytochelatins in plants[J ]. Plant Phys., 122: 793 ～ 801.
[9]Senft AP, Dalton TP, Shertzer HG. 2000. Determining glutathione and glutathione disulfide using the fluorescence probe o-phthalaldehyde[J]. Anal. Biochem., 280: 80～ 86.
[10]Sheller FEC, van Heerwaarden LM, Koevoets PLM, et al. 2000.Derivatization of phytochelatins from Silene vulgaris, induced upon exposure to arsenate and cadmim: Comparison of derivatization with ellman's reagent and monobromobimane[J]. J. Agric. Food Chem., 48:4014～4019.
[11]Stolt JP, Sheller FEC, Bryngelsson T, et al. 2003. Phytochelatin and cadmium accumulation in wheat [ J ]. Environ. Exp. Bot.,49:21～28.
[12]Xiang CB, Wemer BL, Christensen ELM, et al. 2001. The biological functions of glutathione revisited in arabidopsis transgenic plants with altered glutathione levels[J ]. Plant Phys., 126:564～574.
[13]Zenk MH. 1996. Heavy metal detoxification in higher plants - A review[J]. Gene, 179:21～ 30.

[1]	WEI Hai-ying, LI Yi-yang, SHANG Tian-cui, ZHANG Wei. Population structure and quantitative dynamics of Juglans regia individuals generating from branches in different slope directions in Wild Walnut Natural Conservation Area in Xinjiang, China. [J]. Chinese Journal of Ecology, 2023, 42(2): 266-273.
[2]	HUANG Chunxiao, LIU Quanru, DU Yuxuan, HU Yu, HE Yi. Population age structure and quantitative dynamics of Clematis acerifolia, a rare and endangered species endemic to Taihang Mountains. [J]. Chinese Journal of Ecology, 2023, 42(12): 2911-2917.
[3]	. Assemblage structure of larvae and juvenile fish of Changqingsha and Minzhusha in Rugao Section at the lower reaches of the Yangtze River. [J]. Chinese Journal of Ecology, 2022, 41(9): 1778-1786.
[4]	LI Meng-ting, LIU Jin-ling, ZHAO Ben-liang, DENG Xiao-yu, CHEN Hui-ying, TANG Yi-jie. Research progress on characteristics and applications of endogenous cellulase in apple snail. [J]. Chinese Journal of Ecology, 2021, 40(2): 559-567.
[5]	LIU Shi-ling, JIA Hong-yan, CHEN Lin, YANG Bao-guo, LEI Li-qun, PANG Sheng-jiang, ZHANG Pei. Effects of container size and medium formula with biochar addition on the growth of Betula alnoides seedlings. [J]. cje, 2019, 38(9): 2875-2882.
[6]	LIU Shuang-shuang, WANG Ming, DONG Yan-min, WANG Sheng-zhong, HAN Yuan-yuan, CAO Yi-wen, XU Zhi-wei. The influence of hummock microtopography on plant litter decomposition in Carex peat mire. [J]. cje, 2018, 37(1): 95-102.
[7]	JIN Hui, ZHAO Ying, YIN Hang, QIN Li-wu, LIU Li-jie, WANG Chao, JIA Xiang, LI Bing-yan. Quantitative characteristics and dynamic analysis of the endangered species Rhododendron chrysanthum* population in Changbai Mountain. [J]. cje, 2017, 36(11): 3123-3130.
[8]	ZENG Jia-qing, ZHU Jia-xing, WANG Wei, TAO Jian-ping. Population structure and spatial pattern of Alchornea davidii* in different disturbed Karst habitats in Chongqing. [J]. cje, 2016, 35(9): 2313-2320.
[9]	ZHU Yun-jie1,2, GU Dang-en1, WEI Hui1, MU Xi-dong1, LUO Du1, XU Meng1, LUO Jian-ren1, HU Yin-chang1. Age structure and growth characteristics of exotic fish Clarias gariepinus* (Burchell, 1822) in south China. [J]. cje, 2016, 35(6): 1561-1568.
[10]	ZHOU Xing-an1, QIAO Yong-min1,2, WANG sai1, YANG yang1,2*, LIU Xi-xi1. Characteristics of fish assemblages associated with environmental factors in the Lake Erhai. [J]. cje, 2016, 35(6): 1569-1577.
[11]	ZHANG Hua1,2, HUANG Qin1, ZHAO Chuan-yan2, ZHANG Lan1, XU Chun-hua1, KANG Ya-rong1. Spatial pattern analysis of individuals in different ageclasses of Picea crassifolia*. [J]. cje, 2016, 35(5): 1182-1187.
[12]	CAO Yan-min*. Subfossil chironomid assemblages and the related environmental factors in subalpine mires, western Hubei Province. [J]. cje, 2016, 35(5): 1268-1276.
[13]	FANG Miao1,2, XU Meng1, LUO Du1, MU Xi-dong1, GU Dang-en1, HU Yin-chang1, ZHANG Jia-en3, LUO Jian-ren1. Measurable characters, population structure and density effect of Pomacea canaliculata* in different habitats. [J]. cje, 2016, 35(3): 747-754.
[14]	LUO Ling1,2, WANG Zong-ming1, MAO De-hua1, ZHANG Bai1*, LU Chun-yan1,2, MAN Wei-dong1,2. Connotation and differentiation of terminology on main kinds of wetlands in English. [J]. cje, 2016, 35(3): 834-842.
[15]	LI Nan1,2, GAO Zhen-rui1,2, ZHANG Xiao-na1,2, CUI Min-long1. Cloning and expression of a Cdtransport related gene GmNramp1* in soybean. [J]. cje, 2016, 35(12): 3293-3298.