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cje ›› 2004, Vol. ›› Issue (1): 30-33.

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Component changes of humic substances and heavy metal distribution before and after sewage sludge composting

ZHANG Xueying1, ZHOU Shungui1, ZHOU Lixiang1, WONG J. W. C2, WU Qitang 3   

  1. 1. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;
    2. Department of Biology, Hong Kong Baptist University, Hong Kong;
    3. College of Land Resources and Environmental Sciences, South China Agricultural University, Guangzhou, 510064, China
  • Received:2002-10-24 Revised:2003-01-07 Online:2004-02-10

Abstract: Molecular size distribution of humic substance before and after sludge composting was investigated by dialysis and Sephadex Gel-75 chromatography. Cu and Zn in each fractions of different molecular size were also determined. The results showed that after composting, the content of low molecular size fraction with molecular weight cut off <1000 Da in humic substance decreased by 64% while high molecular size fraction with >25000 Da increased by 68%. The evidence from the Gel-75 chromatography also further confirmed that the content of the higher molecular size fraction with >2000 KDa in composted sludge was 2.3 times higher than that of non-composted sludge. In the different fractions of humic substance, Cu was found to be more favorably bound with high molecular size fraction, but Zn was easily associated with low molecular size fraction.

Key words: Alnus, Hippophae nodule, Frankia, Diversity, ARDRA

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