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王锦蛇出生至1月龄白细胞百分比的变化模式及其适应意义

孙嘉珞,高慧清,连丽燕,张志强*   

  1. (安徽农业大学动物科技学院, 合肥 230036)
  • 出版日期:2017-08-10 发布日期:2017-08-10

Variation pattern and adaptive significance of different subtypes of leukocytes in the king ratsnakes (Elaphe carinata) from birth to 30 days of postembryonal period.

SUN Jia-luo, GAO Hui-qing, LIAN Li-yan, ZHANG Zhi-qiang*#br#   

  1. (College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China).
  • Online:2017-08-10 Published:2017-08-10

摘要: 为探究王锦蛇(Elaphe carinata)胚后发育免疫系统的建立过程,为其健康养殖和疾病诊断提供基础生物学数据,在室温为(26±2)℃、相对湿度为70%~80%的条件下,每组5条幼蛇,连续监测了出壳组(0 d)、蜕皮组(出壳后7~10 d),灌喂鸡蛋液10 d组(出壳后20 d)和20 d组(出壳后30 d),其体重、体长、重长比和血液中各型白细胞的百分比的变化情况。结果表明:从0 d至30 d,体重增加了20.37%,但无明显的组间差异(P>0.05);蜕皮组和灌喂10 d组的体长显著高于出壳组(P<0.05);重长比无明显的组间差异(P>0.05);嗜中性粒细胞、嗜碱性粒细胞和淋巴细胞的百分比均无组间差异(P>0.05);单核细胞的百分比出壳组较高,灌喂10 d组较低,但无组间差异(P>0.05),而嗜酸性粒细胞的百分比灌喂10 d组高于出壳组(P<0.05);胚后发育30 d内,王锦蛇的体重和体长持续增加,各型白细胞在出壳时已发育完全,但随发育进程的推进,单核细胞的百分比表现出下降的趋势,而嗜酸性粒细胞的百分比则明显增加,这可能与开口后的食物刺激及基于它们的生活习性而导致的被咬伤的概率增加有关,提示在养殖过程中应先以流质食物灌饲为主,并避免将个体差异较大的幼蛇置于同一蛇箱中。

关键词: 适用性, 干旱指数, 农业干旱评估, 吉林省

Abstract: To explore the establishment of the immune system during postembryonic development, and provide basic biological data for further healthy culture and disease diagnosis, the changes of body mass (BM), body length (BL), the ratio of BM to BL, and the percentages of different subtypes of leukocytes from hatched group (on day 0 after hatching), sloughing group (on day 7-10 after hatching), 10-day group fed with chicken egg fluid (on day 20 after hatching), and 20day group fed with chicken egg fluid (on day 30 after hatching) were measured in the king ratsnakes (Elaphe carinata). The snakes were cultured under (26±2) ℃ indoor temperatures and 70%-80% relative humidity. Five individuals per group were sampled. BM was increased by 20.37% from day 0 to day 30, but no significant group differences were found (P>0.05). BL was longer in both sloughing and 10-day groups than that in the hatched group (P<0.05). There was no significant difference in the ratio of BM to BL among the four groups (P>0.05). No group differences were observed for the percentages of neutrophils, basophils and lymphocytes (all P>0.05). The percentage of monocytes was higher in the hatched group, and lower in the 10-day group, but did not show significant group differences (P>0.05). However, the percentage of eosinophils was significantly higher in the 10-day group than in the hatched group (P<0.05). During the 30-day postembryonic development, BM and BL displayed continuously increased tendencies, and different subtypes of leukocytes occurred before they were hatched. The percentages of monocytes and eosinophils showed reverse tendencies, the former was reduced and the latter was increased from day 0 to day 30. These results may be related to initial food stimulation after feeding and more wounded probability caused by biting each other due to their living habits, suggesting that it is necessary to feed the king ratsnakes with fluid food during early postembryonic stages, and put juvenile snakes of similar body size into the same culture room.

Key words: agricultural drought evaluation, applicability, drought index, Jilin Province