Abstract: Soil macrofauna play an important role in nutrient cycling and energy flow in farmland ecosystems. To study the response of soil macrofauna communities to notillage stover mulch systems, a survey of soil macrofauna community was carried out at the Lishu site of the Chinese Academy of Sciences in April, July and October of 2015. On each sampling visit, soil macrofauna were collected by hand from five types of plot, namely notill for three years without stover mulch plot (NT0), notill for three years with one year of stover mulch (NT1/3), notill for three years with two years of stover mulch plot (NT2/3), notill for three years with three years of stover mulch plot (NT3/3), and a conventional tillage as the control. Within each plot, three subplots (25 cm×25 cm) were selected along the diagonal linetransect. Soil macrofauna were collected from three soil depths (0-5, 5-10, 10-15 cm) in each subplot. A total of 26 soil macrofauna groups belonging to 13 orders, 5 classes and 2 phyla were found during this study. The dominant macrofauna group was Enchytraeidae in the all treatment plots. The individual density, taxon richness, and diversity index of macrofauna were higher in the notillage stover mulch plots than those of conventional tillage plots. The highest individual density of soil macrofauna community was recorded in October for all study plots, whereas the highest of taxon and species diversity were recorded in July. The no tillage stover mulch system can increase the individual density for all soil macrofauna groups. Different soil macrofauna groups differed in response to the increased stover mulching frequency. Compared with conventional tillage, no tillage stover mulch could increase the individual density of saprophagous group.