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竹林金针虫对绿僵菌及其代谢产物的行为反应

叶碧欢,张亚波,舒金平*,吴鸿,王浩杰   

  1. (中国林业科学研究院亚热带林业研究所, 浙江富阳 311400)
  • 出版日期:2016-04-10 发布日期:2016-04-10

Behavioral responses of bamboo wireworm, Melanotus cribricollis to Metarhizium pingshaense and its metabolites.

YE Bi-huan, ZHANG Ya-bo, SHU Jin-ping*, WU Hong, WANG Hao-jie   

  1. (Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China).
  • Online:2016-04-10 Published:2016-04-10

摘要:

绿僵菌(Metarhizium spp.)是地下害虫重要的生防真菌,为了明确绿僵菌对金针虫的驱避作用,本文以平沙绿僵菌WP08菌株为研究对象,通过Y型管等嗅觉生测方法测定了筛胸梳爪叩甲幼虫对绿僵菌分生孢子、非挥发物和挥发性物的行为反应。结果表明:金针虫的选择行为与土壤中平沙绿僵菌分生孢子浓度密切相关,当土壤中绿僵菌孢子浓度达到5×108个·g-1干土时,绿僵菌对金针虫表现出极显著的驱避作用(P<0.001),随着浓度的降低,驱避作用减弱,当浓度达到5×106个·g-1干土时,驱避作用消失;平沙绿僵菌孢子发酵液(浓度为1×108个·mL-1)对金针虫驱避作用极显著(P<0.001),而挥发物对金针虫驱避作用不明显(P>0.05)。本研究结果为揭示绿僵菌驱虫的内在机制提供了重要的基础信息。
 

关键词: 相对权重, 气象因子, 种群数量, 大兴安岭, 落叶松毛虫

Abstract: Metarhizium spp. are very important entomogenous fungi for the biocontrol of soil insects. In order to clarify the repellent effect of Metarhizium spp. against wireworms, we tested the behavioral responses of Melanotus cribricollis wireworm towards the conidium, volatiles and nonvolatiles of Metarhizium pingshaense WP08 using Ytube olfactory bioassay in the present study. The results showed that when the concentration of M. pingshaense WP08 conidium was 5×108 conidia·g-1 dry soil, wireworms were repellent significantly by the conidium (P<0.001), and the repellency was weakened with the decrease of conidium concentration in the soil. There was no repellency against the wireworms when the conidium concentration was 5×106 conidia·g-1 dry soil. The fermentation broth of M. pingshaense WP08 conidium (1×108 conidia·mL-1) showed significant repellency against the wireworms (P<0.001), while the VOCs emitted from M. pingshaense showed no significant repellency against wireworms (P>0.05). Our results provide important basic information of chemical mechanism of fungirepellency against insects.

Key words: larch caterpillar, population quantity, Great Xing’an Mountains, relative weight, meteorological factors