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重金属污染区与非污染区乌苏里风毛菊对铅胁迫的生理抗性

王一峰**,岳永成   

  1. (西北师范大学生命科学学院, 兰州 730070)
  • 出版日期:2014-09-10 发布日期:2014-09-10

Physiological response of Saussurea ussuriensis to Pb stress in Pb polluted and nonpolluted areas.

WANG Yi-feng**, YUE Yong-cheng   

  1. (College of Life Sciences Northwest Normal University, Lanzhou 730070, China)
  • Online:2014-09-10 Published:2014-09-10

摘要:

以分布于甘肃徽县重金属污染区和甘南藏族自治州非污染区的乌苏里风毛菊(Saussurea ussuriensis)为研究对象,采用水培试验方法,通过测定和比较其对不同浓度铅胁迫的生理指标,研究了重金属污染区与非污染区乌苏里风毛菊对铅胁迫的生理抗性响应,探究风毛菊属植物对重金属铅的抗性生态适应机制。结果表明:在不同浓度铅胁迫处理下,污染区乌苏里风毛菊叶绿素含量呈现先上升后下降的趋势,而非污染区乌苏里风毛菊叶绿素含量均呈现下降趋势;污染区与非污染区两个居群乌苏里风毛菊抗氧化酶系的酶活性均呈现先升高再降低的趋势,但污染区乌苏里风毛菊抗性相对较强;污染区与非污染区两个居群乌苏里风毛菊MDA含量均升高,非污染区乌苏里风毛菊MDA含量的上升趋势明显大于污染区种;两个居群乌苏里风毛菊可溶性蛋白含量均减少,其中非污染区乌苏里风毛菊可溶性蛋白含量的减小趋势明显大于污染区种。不同浓度铅胁迫处理下,污染区乌苏里风毛菊这些变化表明其已经产生了适应性抗性进化,形成了重金属抗性生态型。

 

关键词: 水环境, 水质, 模型模拟, 河流, 生态工程

Abstract:

A hydroponics was used to study the physiological response of Saussurea ussuriensis. to Pb stress. Two samples of S. ussuriensis were from a Pbpolluted area in Huixian and a nonpolluted area in Gannan, respectively. The aim was to explore the ecological adaptation mechanism of the two S. ussuriensis populations to Pb stress. The results showed that, at different Pb concentrations, the chlorophyll content of S. ussuriensis from the polluted area increased first and then decreased, while that from the nonpolluted area showed a decreasing trend. The antioxidase enzyme activity of both S. ussuriensis populations increased first and then decreased, but the resistance of S. ussuriensis from the polluted area was stronger. The MDA contents of both S. ussuriensis populations increased with Pb concentrations, but it was significantly higher in the nonpolluted group than in the polluted group. The soluble protein contents of both S. ussuriensis populations decreased, being significantly higher in the polluted group than in the nonpolluted group. The variations in physiological indexes of S. ussuriensis from the polluted area indicated an adaptive evolution, forming a heavy metalresistance ecotype.
 

Key words: water quality, river, model simulation, water environment, ecological engineering