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不同高温水平对虾夷扇贝存活率、耗氧率和体腔液免疫酶活力的影响

郝振林,唐雪娇,丁君,贲月,常亚青**   

  1. (大连海洋大学农业部北方海水增养殖重点实验室, 辽宁大连 116023)
  • 出版日期:2014-06-10 发布日期:2014-06-10

Survival rate, oxygen consumption rate and immune enzymetic activity of Mizuhopecten yessoensis at high temperature.

HAO Zhen-lin, TANG Xue-jiao, DING Jun, BEN Yue, CHANG Ya-qing**   

  1. (Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture of China, Dalian Ocean University, Dalian 116023, Liaoning, China)
  • Online:2014-06-10 Published:2014-06-10

摘要:

实验室内营造不同升温模式,研究了2种规格(10和46月龄)虾夷扇贝(Mizuhopecten yessoensis)的存活率、耗氧率及体腔液免疫酶活力对不同高温水平(20、22、24、26、28 ℃)的响应,分析了2种规格虾夷扇贝在不同升温模式下的耐高温能力。实验设计了缓慢升温和快速升温2种升温模式。缓慢升温模式,将在15 ℃暂养的2种规格扇贝分别驯化到20、22、24、26和28 ℃温度水平(升温幅度为1 ℃·d-1),暂养14 d后进行实验。快速升温模式,将在15 ℃暂养的2种规格扇贝分别快速升温到20、24及28 ℃温度水平(升温幅度为4 ℃·d-1),暂养1 d后进行实验。结果表明:缓慢升温模式时,随温度升高,扇贝存活率逐渐降低,28 ℃时,46和10月龄贝存活率最低分别为96.7%和77.3%,此外,26~28 ℃时,各实验处理组,46月龄贝存活率显著高于10月龄贝(P<0.05);2种规格扇贝耗氧率随温度升高均呈先上升后下降趋势;15~24 ℃时,10月龄贝的耗氧率显著高于46月龄贝(P<0.05);28 ℃时,10和46月龄贝的耗氧率降至最低,分别为0.527和0.482 mg·g-1·h-1;另外,扇贝的体腔液免疫酶活力随温度升高也均呈现先上升后下降的变化趋势,且在相同升温模式下,46月龄贝的免疫酶活力显著高于10月龄贝(P<0.05)。综上,规格较大的虾夷扇贝在不同高温水平时均表现出较强的耐高温能力。
 

关键词: 生态系统生态学, 人类福祉, 生态红线, 实证研究, 供给和消费

Abstract: Different temperature heating modes were designed to study the response of twosize scallops (10 months and 46 months old) at different high temperature levels (20, 22, 24, 26, 28 ℃) and their capabilities in resisting high temperature under different heating modes (slow heating and abrupt heating), and the survival rate, oxygen consumption rate and immune enzyme activity were investigated in this paper. In the slow heating pattern, scallops reared in 15 ℃ were acclimatized to the different temperature levels 20, 22, 24, 26 and 28 ℃ (temperature increased 1 ℃ per day), and then maintained for 15 days. In the abrupt heating pattern, the rearing temperature was increased from 15 to 20, 24, and 28 ℃ at a rate of 4 ℃ per day, and maintained for 1 day. The results showed that the survival rates of scallops decreased with increasing the temperature under the slow heating mode, and the survival rates of 46 and 10monthold scallops were 96.7% and 77.3% at 28 ℃ respectively. The 46monthold scallops had a significantly higher survival rate than the 10monthold ones when they were exposed to each temperature treatment (P<0.05). The twosize scallops had similar oxygen consumption changes under the test temperature treatments, firstly the oxygen consumption increase, followed by a quick decrease with rising the water temperature. The 10monthold scallops had a significantly higher oxygen consumption rate than the 46monthold ones when they exposed to 15-24 ℃ temperature (P<0.05). When water temperature reached 28 ℃, the oxygen consumption rates of 46 and 10monthold scallops both dropped to the lowest with values at 0.482 and 0.527 mg·g-1·h-1, respectively. The related immune enzyme activities of the twosize scallops also had a similar change at the variable temperatures, however, the 46monthold scallops had a significantly higher value for the immune enzyme activities than the smallsize ones when they were in the same treatment (P<0.05). In a word, our results suggested that the larger scallops had a stronger capability in resisting high temperature compared with smaller ones under different heating modes.

Key words: humanity well-being, supply and consumption, ecological red line., ecosystem ecology, empirical research