复合微生物菌剂对石油污染土壤的修复

生态学杂志

复合微生物菌剂对石油污染土壤的修复

雒晓芳¹,陈丽华^1**,王冬梅¹,李瑛娟²,马莉²

(¹西北民族大学实验中心，兰州 730030; ^2西北民族大学化工学院，兰州 730030)

出版日期:2013-09-10 发布日期:2013-09-10

Remediation of oil-contaminated soil by compound microbial preparation.

LUO Xiao-fang¹, CHEN Li-hua^1**, WANG Dong-mei¹, LI Ying-juan², MA Li^2

(¹Center of Experiment, Northwest University for Nationality, Lanzhou 730030, China; ^2Chemical Engineering Institute, Northwest University for Nationality, Lanzhou 730030, China)

Online:2013-09-10 Published:2013-09-10

摘要/Abstract

摘要：

利用分离的5株石油降解菌及其复合菌剂对石油污染土壤进行了实验室模拟与现场修复研究。结果表明：经过48 d的修复，复合菌剂组的降解率可达84%以上，显著高于单一株菌; 各菌剂降解能力表现为H>A6>F2>A5>D4>F1; 降油率在10 d左右增长幅度最大，20 d之后逐渐减缓; 复合菌剂组的脱氢酶活性明显高于单一株菌组; 土壤微生物数量、脱氢酶活性和降油率三者之间呈显著的相关性；随着时间的变化，石油类化合物、饱和烃和芳香烃都有明显的降解，其中饱和烃的降解速度最快，非烃和沥青质最慢。

Abstract: Five strains of separated oil-degrading bacteria and their compound preparations were used to study their remediation effects on oil-contaminated soil by laboratory simulation and field repair. After treated with compound preparations for 48 days, the oil-degrading rate was above 84%, obviously higher than that treated with single strain. The biodegradability of all strains was in the order of H>A6>F2>A5>D4>F1. The oil-degrading rate increased sharply near 10 days, but gradually slowed down after 20 days. The dehydrogenase activity of compound preparations was higher than that of single strain. There existed significant correlations between the microbial population size, dehydrogenase activity, and oil-degrading rate. With the time changed, the oil-based compounds, saturated hydrocarbons, and aromatic hydrocarbons had obvious degradation, among which, saturated hydrocarbon had the fastest degradation rate, while nonhydrocarbon and asphaltene had the slowest one.

雒晓芳1,陈丽华1**,王冬梅1,李瑛娟2,马莉2. 复合微生物菌剂对石油污染土壤的修复[J]. 生态学杂志.

LUO Xiao-fang1, CHEN Li-hua1**, WANG Dong-mei1, LI Ying-juan2, MA Li2. Remediation of oil-contaminated soil by compound microbial preparation.[J]. cje.