白术内生细菌分离与促生活性

生态学杂志

白术内生细菌分离与促生活性

阳湖荣,陈飘雪,戴传超^*

(南京师范大学生命科学学院，江苏省微生物与功能基因组学重点实验室，江苏省微生物资源产业化工程技术研究中心， 南京 210023)

出版日期:2019-12-10 发布日期:2019-12-10

Isolation and plant growthpromoting ability of endophytic bacteria in Atractylodes macrocephalaKoidz.

YANG Hu-rong, CHEN Piao-xue, DAI Chuan-chao^*

(Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China).

Online:2019-12-10 Published:2019-12-10

摘要/Abstract

摘要： 为筛选促进药用植物活性成分积累的内生细菌，本研究采用纯培养分离白术可培养内生细菌，通过16S rDNA鉴定，并检测其促生活性。结果表明：从白术体内分离出49株内生细菌，包含11个属，芽孢杆菌属（Bacillus）和假单胞菌属（Pseudomonas）是白术体内的优势菌属；分离株中，分别有10、25、19株具有固氮、溶磷和解钾的能力，35株能够合成吲哚乙酸；其中，Bacillus endophyticus AMR83同时具有固氮、溶磷、解钾和合成吲哚乙酸的潜力，且该菌株能促进白术组培苗根部生长并促进根部挥发油含量提高100.55%，调节白术活性成分的配比。综上，AMR83可作为一株提高白术挥发油产量、改善药材品质的优良菌株。

关键词: 定量评价技术, 数值模拟, 地面气象观测, 城市热环境, 热红外遥感

Abstract: To screen endophytic bacteria that promote the accumulation of bioactive ingredients in medicinal plants, endophytic bacteria residing Atractylodes macrocephala were cultured by pure cultured method and identified by 16S rDNA sequencing. The biochemical characteristics and effects on host plant growth were analyzed. Results showed that a total of 49 endophytic bacteria were isolated from A. macrocephala, including 11 genera. Bacillus and Pseudomonas were the dominant genera. Among the isolated strains, there were 10, 25, 19 strains with ability of nitrogen fixation, phosphorus solubilization and potassium dissolving, respectively. 35 strains were able to synthesize indole acetic acid. Bacillus endophyticus AMR83, a stain with all the above biological characteristics, can promote plant root growth, increase the volatile oil content inA. macrocephala roots by 100.55%, and adjust the proportion of active ingredients. The results suggested that AMR83 could be used as an endophyte to improve the yield of volatile oil and the quality of crude drug.

Key words: quantitative evaluation techniques, thermal infrared remote sensing, urban thermal environment, ground meteorological observation, numerical simulation.

阳湖荣,陈飘雪,戴传超. 白术内生细菌分离与促生活性[J]. 生态学杂志.

YANG Hu-rong, CHEN Piao-xue, DAI Chuan-chao. Isolation and plant growthpromoting ability of endophytic bacteria in Atractylodes macrocephalaKoidz.[J]. cje.

[1]	苏王新, 张刘宽, 常青. 基于MSPA的街区蓝绿基础设施格局及其热缓解特征 [J]. 生态学杂志, 2022, 41(6): 1173-1181.
[2]	孙玫玲,张赛,马京津. 津秦高速铁路沿线风况特征 [J]. 生态学杂志, 2019, 38(11): 3487-3495.
[3]	张雷1,张鹏1,王华2,周新华3,虞木奎1,吴统贵1*. 沿海水杉防护林带三维结构参数模型 [J]. 生态学杂志, 2017, 36(4): 1127-1133.
[4]	孙琳丽1,侯琼2,赵慧颖3*,李思慧1,赵放3,邹艳东1. 河套灌区不同强度低温冷害对玉米生物量累积和产量的影响 [J]. 生态学杂志, 2016, 35(1): 17-25.
[5]	荣飞;翟保平;姜玉英. 小麦条锈病典型越夏区孢子传输的数值模拟 [J]. 生态学杂志, 2008, 27(12): 2099-2104 .