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• 研究报告 • 上一篇    下一篇

不同温度下刺参肠道菌群结构与功能

全紫娇,高萍萍,张杨,王荦,丁君*常亚青*   

  1. (大连海洋大学农业农村部北方海水增养殖重点实验室, 辽宁大连 116023)
  • 出版日期:2019-09-10 发布日期:2019-09-10

Bacterial community and function in the intestinal tracts of sea cucumber (Apostichopus japonicus) at different temperatures.

QUAN Zi-jiao, GAO Ping-ping, ZHANG Yang, WANG Luo, DING Jun*, CHANG Ya-qing*   

  1. (Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs at Dalian Ocean University, Dalian 116023, Liaoning, China).
  • Online:2019-09-10 Published:2019-09-10

摘要: 采用MiSeq高通量测序技术,研究了温度为10、16、21、26 ℃条件下,养殖刺参(Apostichopus japonicus)肠道菌落结构。结果表明:刺参肠道内优势菌门主要为拟杆菌门(Bacteroidetes)、疣微菌门(Verrucomicrobia)、变形菌门(Proteobacteria);优势菌属主要为RubritaleaLutibacter和弧菌属(Vibrio);Lutibacter与弧菌属在10 ℃组的比例最大,Rubritalea在26 ℃时所占比例最大,这些优势菌群在不同温度下占刺参肠道菌群的比例互不相同,说明刺参的肠道菌群与其生长温度之间有一定联系;不同温度下刺参的肠道菌群存在特殊菌属,10 ℃组的特别菌属为LuteolibacterColwellia,21 ℃组特有的菌属为珊瑚微球菌(Coraliomargarita),26 ℃组特别菌属为发光杆菌属(Photobacterium),夏眠组的特有菌属最多,包括鞘脂单胞菌属(Sphingomonas)、气单胞菌属(Aeromonas)和苯基杆菌属(Phenylobacterium);鞘脂单胞菌属是动物病原体且气单胞菌属对刺参有一定毒力,是刺参高温下存活率低的原因之一;各样品中菌群经COG及KEGG数据库分析,共注释到24组COG功能分类,主要分布在氨基酸转运与代谢、转录、细胞壁/膜/包膜生物发生;41条KEGG信号通路,集中分布在氨基酸转运与代谢、碳水化合物代谢、膜转运、复制和修复;刺参肠道微生物基因功能大多集中在氨基酸代谢、碳水化合物代谢、能量代谢与物质转运、复制和修复方面,这些功能与刺参正常生长密切相关,在10 ℃组功能丰度最低。

关键词: 长汀县, 生态脆弱性, 评价, 等级指数, 遥感

Abstract: MiSeq high-throughput sequencing technology was used to investigate the intestinal colony bacterial community structure of Apostichopus japonicus at 10, 16, 21, and 26 ℃. The results showed that bacterial phylotypes in all groups are closely related at phylum level, with Bacteroidetes, Verrucomicrobia, and Proteobacteria being the dominant phyla. The dominant genera in the intestinal tract of sea cucumber were Rubritalea, Lutibacter, and Vibrio. Lutibacter and Vibrio had the largest proportion at 10 ℃, and Rubritalea had the largest proportion at 26 ℃. The proportion of these dominant bacteria in the gut microbiota at different temperatures is different, indicating that there is certain relationship between the intestinal flora of sea cucumber and its growth temperature. Particular bacteria in the intestinal flora of sea cucumber existed at different temperatures. The bacteria in the 10 ℃ group were Luteolibacterand Colwellia, the bacteria in the 21 ℃ group was Coraliomargarita, and in the 26 ℃ group was Photobacterium. The aestivation group had the most endemic bacteria, including Sphingomonas,Aeromonasand Phenylobacterium. Sphingomonas is an animal pathogen and Aeromonas has a certain virulence to sea cucumber, which is associated with low survival rate of sea cucumber at high temperature. Results of COG and KEGG annotation analysis revealed 24 COG functions and 41 KEGG pathways, and their functions belonged to amino acid transport and metabolism, carbohydrate transport and metabolism, and membrane transport. Most of the gut microbial gene functions of sea cucumber are concentrated in amino acid metabolism, carbohydrate metabolism, energy metabolism and substance transport, replication, and repair. These functions are closely related to the growth of sea cucumber. The functional abundance was the lowest at 10 ℃ treatment.

Key words: Changting County, ecological vulnerability, assessment, level index, remote sensing.