Cd胁迫下不同耐性豆科植物根内活性氧产生与基因组DNA多态性变化

生态学杂志 ›› 2012, Vol. 31 ›› Issue (09): 2330-2336.

Cd胁迫下不同耐性豆科植物根内活性氧产生与基因组DNA多态性变化

张芬琴¹,张红晓²,沈振国^3**

（¹河西学院农业与生物技术学院，甘肃张掖 734000； ²河南科技大学农学院，河南洛阳 471003； ³南京农业大学生命科学学院，南京 210095）

出版日期:2012-09-10 发布日期:2012-09-10

Reactive oxygen production and genome DNA polymorphism change in root tip tissues of two leguminous species with different tolerance under Cd stress.

ZHANG Fen-qin¹, ZHANG Hong-xiao², SHEN Zhen-guo^3**

(¹College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, Gansu, China; ²College of Agriculture, Henan University of Science and Technology, Luoyang 471003, Henan, China; ³College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China)

Online:2012-09-10 Published:2012-09-10

摘要/Abstract

摘要： 采用溶液培养法，研究了镉胁迫对绿豆和箭舌豌豆幼苗根基因组DNA多态性和活性氧产生的影响。结果表明：Cd胁迫降低了2种植物根尖基因组DNA模板的稳定性，导致DNA多态性发生了变化，包括RAPD谱带的增加、缺失及其荧光强度的改变；同时，Cd胁迫抑制了根的伸长生长，促进了根内超氧阴离子（O₂^-_·）产生和过氧化氢（H₂O₂）积累；Cd胁迫下2种植物根的伸长与其根内O₂^-·产生、H₂O₂积累以及DNA多态性呈负相关，DNA多态性与Cd胁迫下H₂O₂积累呈正相关。

关键词: 卤代甲烷, 排放通量, 自然释放, 盐沼, 海岸带

Abstract: The effects of cadmium (Cd) on the reactive oxygen productions and genome DNA polymorphism changes in roots of Phaseolus aureus Roxb. cv. VC3762 (Cdsensitive species) and Vicia sativa L. cv. 333/A (Cdresistant species) seedlings were investigated using hydroponic solution culture. The results showed that Cd stress decreased the genomic template stability and induced the changes of the genome DNA polymorphism in the root tips of the two species, manifesting the variation of band intensity, loss of normal bands, and appearance of new bands. Meanwhile, Cd stress inhibited the root growth and increased the production of O₂^-· and the accumulation of H₂O₂ in the roots. The root length of the two species under Cd stress was negatively correlated with the production of O₂^-·, accumulation of H₂O₂, and genome DNA polymorphism in root tip tissues, and the genome DNA polymorphism was positively correlated with the accumulation of H₂O₂ under Cd stress.

Key words: methyl halides, emission fluxes, natural emission, salt marsh, coastal zone.

张芬琴1,张红晓2,沈振国3**. Cd胁迫下不同耐性豆科植物根内活性氧产生与基因组DNA多态性变化[J]. 生态学杂志, 2012, 31(09): 2330-2336.

ZHANG Fen-qin1, ZHANG Hong-xiao2, SHEN Zhen-guo3**. Reactive oxygen production and genome DNA polymorphism change in root tip tissues of two leguminous species with different tolerance under Cd stress.[J]. cje, 2012, 31(09): 2330-2336.

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