药用植物华中五味子的种群遗传多样性及遗传结构

生态学杂志

药用植物华中五味子的种群遗传多样性及遗传结构

闫伯前^1,2,4;王艇¹;胡理乐³

¹中国科学院武汉植物园，武汉 430074;²北京农学院，北京 102206;³中国环境科学研究院，北京 100012;⁴中国科学院研究生院，北京　100039

收稿日期:2008-11-02 修回日期:1900-01-01 出版日期:2009-05-10 发布日期:2009-05-10

Population genetic diversity and structure of Schisandra sphenanthera, a medicinal plant in China.

YAN Bo-qian^1,2,4;WANG Ting¹;HU Li-le³

¹Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;²Beijing University of Agriculture, Beijing 102206, China;³Chinese Research Academy of Environmental Sciences, Beijing 100012, China;⁴Graduate University of Chinese Academy of Sciences, Beijing 100039, China

Received:2008-11-02 Revised:1900-01-01 Online:2009-05-10 Published:2009-05-10

摘要/Abstract

摘要： 华中五味子（Schisandra sphenanthera）是著名的药用植物，具有悠久的药用历史和巨大的开发潜力。为了有效评估、利用和保护华中五味子资源，应用自主开发的9对SSR引物研究了华中五味子自然种群的遗传多样性与遗传结构。结果表明：在10个采样种群中，共检测到58个等位基因，平均预期杂合度H_E为0.528，平均观察杂合度H_O为0.519，较大的连续种群保持了较高的遗传多样性，而小种群的遗传多样性则相对较低;华中五味子总体表现为显著的杂合子缺失，内繁育系数F_IS为0.042;种群间总的遗传分化系数F_ST为0.108，两两种群间分化显著;贝叶斯聚类结果把10个采样种群按遗传组成分为江南组和江北组2组，长江所形成的特殊地理屏障对华中五味子江南和江北地区间较高的遗传分化造成了影响。

关键词: 硒, 圆叶决明, 植株生长, 养分含量, 固氮能力

Abstract: Schisandra sphenanthera has been used as a traditional medicinal plant, possessing a tremendous potential for commercial development. Population genetic information on S. sphenanthera will be helpful for its evaluation, utilization and conservation. Genetic diversity and structure in ten natural populations of the species were analysed by using nine SSR markers developed by the authors. A total of 58 alleles were detected, and the mean expected heterozygosity (HE) and observed heterozygosity (HO) were 0528 and 0519, respectively. Large continuous populations maintained relatively high degree of genetic diversity, whereas small populations exhibited low genetic diversity. In S. sphenanthera, the estimated level of inbreeding was significant with the FIS of 0042. The population genetic differentiation was 0108. Across all populations, each pairwise comparison of genetic differentiation as meaured by FST was significant. Bayesian clustering analysis revealed two genetic clusters: south and north populations drew by the Yangtse River. The special natural barrier and subsequent recolonization play a role in the formation of population genetic structure in S. sphenanthera.

Key words: Selenium, Chamaecrista rotundifolia, Growth, Nutrient content, Nitrogen-fixing ability

闫伯前;王艇;胡理乐. 药用植物华中五味子的种群遗传多样性及遗传结构[J]. 生态学杂志.

YAN Bo-qian^1,2,4;WANG Ting¹;HU Li-le³. Population genetic diversity and structure of Schisandra sphenanthera, a medicinal plant in China.[J]. cje.

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