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土壤镉污染对大蒜幼苗生长及根系抗氧化系统的影响

赵胡1;郑文教2;陈杰1   

  1. 1阜阳师范学院生物系, 安徽阜阳 236041;2厦门大学生命科学
    学院, 福建厦门 361005
  • 收稿日期:2007-08-20 修回日期:1900-01-01 出版日期:2008-05-10 发布日期:2008-05-10

Effects of soil cadmium pollution on Allium sativum seedling growth and root antioxidant system.

ZHAO Hu1; ZHENG Wen-jiao2; Chen Jie1   

  1. 1Department of Biology, Fuyang Teachers College, Fuyang 236041, Anhui,
    China;2School of Life Sciences, Xiamen University, Xiamen 361005, Fujian,
    China
  • Received:2007-08-20 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10

摘要: 通过土培实验研究了镉污染胁迫对大蒜幼苗生长及根系抗氧化系统的影响。结果表明:与对照组比较,土壤低浓度镉对大蒜幼苗生长略有促进作用,单株鲜质量、干质量、根长及地上部分高度略有增加,而根系活力、可溶性蛋白含量、超氧化物坡化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性显著升高,抗氧化防御酶系统仍保持平衡,表现在膜脂质过氧化产物(MDA)含量与对照组无明显差异;但随着土壤镉浓度的进一步增加,大蒜平均单株鲜质量、干质量、平均最大根长及地上部分高度均显著降低,同时,根系活力和可溶性蛋白含量增幅逐渐减弱,抗氧化防御酶系统平衡受到破坏,根系MDA含量显著上升。大蒜对镉吸收积累主要在根部,而向地上部分转运较少。

关键词: 昆虫学, RAPD技术, 分子遗传标记

Abstract: With soil culture experiment, this paper studied the effects of Cd on the seedling growth and root antioxidant system of Allium sativum roots. The results showed that under lower concentrations (<5 mg·L-1) of Cd, the average fresh and dry mass per plant, root length and aboveground height increased slightly, root vigor, soluble protein content, SOD, POD and CAT activities increased obviously, and root antioxidant enzyme system still kept balance, reflecting in the little difference of root MDA content with the control. However, when the Cd concentration was higher than 5 mg·L-1, the average fresh and dry mass per plant, root length and aboveground height decreased markedly, the increments of root vigor and soluble protein content abated, the balance of antioxidant enzyme system was broken, and the MDA content increased obviously. The study also showed the absorbed Cd by A. sativum was mainly accumulated in roots, and only small amount of Cd was transfered to the leaves.

Key words: Entomology, RAPD technique, Molecular genetic markers