不同电子受体下铁还原细菌异化还原Fe(III)性质及菌群特征

生态学杂志

不同电子受体下铁还原细菌异化还原Fe(III)性质及菌群特征

覃海华,忻宏蔚,刘洪艳^*

（天津科技大学海洋与环境学院，天津 300457）

出版日期:2018-06-10 发布日期:2018-06-10

Characteristics of Fe(III) reduction and microbial community of Fe(III) reducing bacteria under different electron acceptors.

QIN Hai-hua, XIN Hong-wei, LIU Hong-yan^*

(College of Marine Science and Environment, Tianjin University of Science & Technology, Tianjin 300457, China).

Online:2018-06-10 Published:2018-06-10

摘要/Abstract

摘要： 将渤海沉积物进行厌氧培养，富集异化Fe(III)还原混合菌群。在不同电子受体下，分析铁还原菌群异化还原Fe(III)性质。以柠檬酸铁和氢氧化铁为电子受体培养体系，在培养12 h时，累积Fe(II)浓度分别为（100.67±0.75）和（53.24±3.63） mg·L^-1；当培养60 h时，累积Fe(II)浓度达到（118.95±1.47）和（119.74±3.96）mg·L^-1。这表明可溶性与不可溶性电子受体能够显著影响细菌异化Fe(III)还原过程，而对累积Fe(III)还原量影响不明显。通过高通量测序技术，分析不同电子受体下的异化Fe(III)还原混合菌群多样性与优势菌组成。菌群多样性分析表明，以柠檬酸铁和氢氧化铁为电子受体时，菌群多样性Shannon指数分别是3.40和3.11，较对照组（Shannon指数2.07）高，表明培养体系中加入Fe(III)能显著提高铁还原混合菌群多样性。异化Fe(III)还原混合菌群在不同电子受体下优势菌主要是Clostridium_sensu_stricto和Romboutsia，属于梭菌目Clostridiales，这表明梭菌是参与Fe(III)还原的优势菌。

关键词: Thornthwaite Memorial模型, 气候生产潜力, 云南省, Miami模型

Abstract: Sea sediments collected from Bohai were anaerobically enriched to obtain dissimilatory Fe(III)reducing bacteria. Characteristics of dissimilatory Fe(III) reduction by Fe(III)reducing bacteria were investigated under different electron acceptors. After fermentation for 12 h, the Fe(II) concentration of culture was (100.67±0.75) and (53.24±3.63) mg·L^-1 respectively, using ferric citrate and ferric hydroxide as electron acceptors. After 60 h, the Fe(II) concentration of culture was (118.95±1.47) and (119.74±3.96) mg·L^-1 respectively. The results indicated that soluble and insoluble electron acceptors could significantly affect Fe(III) reduction processes, but had no significant effect on the accumulation of Fe(II). Diversity of mixed culture and composition of dominant bacteria under different electron acceptors were analyzed by high throughput sequencing. Shannon diversity index of the bacterial community was 3.40 and 3.11 using ferric citrate and ferric hydroxide as electron acceptors, respectively, being higher than that of control group (with Shannon index 2.07). Such a result suggested that cultivation system with Fe(III) addition could significantly enhance the diversity of mixed culture. The dominant populations in the Fe(III)reducing bacteria were Clostridium_sensu_strictoand Romboutsia, belonging to Clostridiales, indicating that Clostridiales were the dominant bacteria in the mixed culture under Fe(III)reducing conditions.

Key words: Miami model, Thornthwaite Memorial model, climatic potential productivity, Yunnan Province.

覃海华,忻宏蔚,刘洪艳. 不同电子受体下铁还原细菌异化还原Fe(III)性质及菌群特征[J]. 生态学杂志.

QIN Hai-hua, XIN Hong-wei, LIU Hong-yan. Characteristics of Fe(III) reduction and microbial community of Fe(III) reducing bacteria under different electron acceptors.[J]. cje.

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