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温水浸种和IAA溶液浸种对当归种子萌发特性的影响

肖生旺1,方向文2,蒋志荣1,3*,程瑶1,马雨婷1#br#   

  1. (1甘肃农业大学林学院, 兰州 730070; 2兰州大学干旱与草地生态教育部重点实验室, 兰州 730000; 3甘肃农村发展研究院, 兰州 730070)
  • 出版日期:2017-05-10 发布日期:2017-05-10

Effects of warm water soaking and IAA soaking on germination of Angelica sinensis seeds.

XIAO Sheng-wang1, FANG Xiang-wen2, JIANG Zhi-rong1,3*, CHENG Yao1, MA Yu-ting1#br#   

  1. (1College of Forestry, Gansu Agricultural University, Lanzhou 730070, China; 2State Key Laboratory of Grassland Agroecosystems, Lanzhou University, Lanzhou 730070, China; 3Gansu Rural Development Research Institute, Lanzhou 730070, China).
  • Online:2017-05-10 Published:2017-05-10

摘要: 为了探索当归种子的休眠机制和提高其萌发率,本文以药用植物当归的种子为研究对象,分析了不同温度的水浸种不同时间和不同浓度3吲哚乙酸(IAA)溶液浸种不同时间后对当归种子萌发率的影响。结果表明:60 ℃水浸种2 min萌发率最高,为78%,是对照的2.5倍;0.05 g·L-1 IAA溶液浸种3 h萌发率最高,为80%,是对照的1.7倍;用30、35、40、45、50、55、60、65、70 ℃ 9个温度的水浸种1、2 min的萌发率与水温的相关性不显著,浸种5、10 min相关性显著,浸种15 min相关性极显著;用0.01、0.02、0.03、0.04、0.05、0.06、0.07、0.08、0.09、0.1 g·L-1 10个浓度的IAA溶液浸种1、3、6、12 h的萌发率与溶液浓度的相关性均不显著;水浸种提高种子萌发率可能与改变种子种皮透性有关,IAA溶液浸种提高种子萌发率可能与打破休眠种子的休眠有关;60 ℃水浸种2 min和0.05 g·L-1 IAA溶液浸种3 h均可显著提高当归种子的萌发率;IAA溶液为有机溶剂,污染环境,在实际生产中建议多使用水浸种法为宜。

关键词: 黄化突变体, 光合作用, 叶绿素含量, 番茄

Abstract: In order to explore dormancybreaking mechanism and increase germination percentage, the effects of different temperatures of water treatments and different concentrations of IAA (indole acetic acid) on the germination process of the seeds of Angelica sinensis were studied. The results showed that soaking seeds with IAA 0.05 g·L-1 for 3 h and soaking seeds with water 60 ℃ for 2 min had the maximum germination percentages (78% and 80%, respectively), being 1.7  and 2.5 times as high as that of the control. When soaking seeds with water 30, 35, 40, 45, 50, 55, 60, 65, 70 ℃ for 1  and 2 min, there was no significant correlation between germination percentages and temperatures, but a significant correlation was observed for 5, 10 and 15 min. There was no significant correlation between germination percentage and concentration when soaking seeds 1, 3, 6, 12 h with IAA 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1 g·L-1. Soaking seeds with water could improve seed germination percentage, which might relate to permeability of seed coat; however, IAA could improve seed germination percentage, which might be involved in breaking seed dormancy. Consequently, soaking seeds with water 60 ℃ for 2 min and soaking seeds with IAA 0.05 g·L-1 for 3 h could improve the germination percentages of A. sinensis seeds. Compared with water, IAA pollutes the environment easily. So, soaking seeds with water is an advisable method in actual production.

Key words: photosynthesis., tomato, chlorophyll-deficient mutant, chlorophyll content