Abstract: Little information is available on planting technology of Dendrobium officinale, a precious medicinal herb endangered in China. This paper studied the screening of seedling cultivation matrix, the effects of epiphyted tree species and epiphytic position and forest crown density on D. officinale growth and the influence of D. officinale planting on the growth of epiphyted trees. The results showed that: (1) A mixture of pine bark, peanut hull and Acacia mangium bark at volumetric ratio of 1:1:1 was an ideal matrix; (2) Different tree species and epiphytic position had significant effects on the height, stem diameter, and biomass of D. officinale, but they did not show interactive effects. The comprehensive evaluation showed that Santalum album, Acacia mangium and Dalbergia odorifera were superior to Dimocarpus longgana and Averrhoa carambola; (3) The most favorable forest canopy density and light intensity for epiphytic colonization were 30%-50%, and about 62.5% of open space, respectively; (4) The epiphytic colonization of D. officinale significantly increased the diameter of epiphyted trees. Overall, pine bark+peanut hull+Acacia mangium bark as matrix and epiphytic colonization on middleheight trunk of Santalum album or Dalbergia odorifera with 30%-50% of canopy density is an encouraging agroforestry model for planting D. officinale in south China.