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横坑切梢小蠹对植物挥发物的电生理和行为反应

王红伟1,杨伟1**,杨桦1,杨春平1,朱晓庆1,刘峰建1,蒋丽华2,山春燕2   

  1. (1四川农业大学森林保护省级重点实验室, 四川雅安 625014; 2四川省泸定县林业局, 四川泸定 626100)
  • 出版日期:2014-05-10 发布日期:2014-05-10

Electroantennogram and behavioral responses of Tomicus minor (Hartig) (Coleoptera: Scolytidae) to plant volatiles.

WANG Hong-wei1, YANG Wei1**, YANG Hua1, YANG Chun-ping1, ZHU Xiao-qing1, LIU Feng-jian1, JIANG Li-hua2, SHAN Chun-yan2   

  1. (1Provincial Laboratory of Forest Conservation, Sichuan Agricultural University, Ya’an 625014, Sichuan, China; 2Forestry Bureau of Luding County, Luding 626100, Sichuan, China)
  • Online:2014-05-10 Published:2014-05-10

摘要:

为筛选出适宜配制横坑切梢小蠹(Tomicus minor Hartig)引诱剂的有效化合物,测定了横坑切梢小蠹雌、雄成虫对寄主云南松主要挥发性化合物的触角电位(EAG)和行为反应。结果表明:与对照相比,在10 g·L-1浓度刺激下所测试的9种植物挥发物都能引起横坑切梢小蠹雌、雄虫触角的EAG反应,且触角对不同挥发物的EAG反应差异显著(P<0.05),其中雌、雄虫对(+)-α-蒎烯、(+)-柠檬烯、(+)-3-蒈烯、β-蒎烯和异松油烯的EAG反应较强;在剂量反应试验中,雌、雄虫的EAG反应都随着(+)-α-蒎烯、(+)-柠檬烯、(+)-3-蒈烯和β-蒎烯浓度的增加而增强,当浓度增加到10 g·L-1时,EAG反应最强;而雌、雄虫对异松油烯的EAG反应在1 g·L-1最强;除(+)-柠檬烯没有明显的定向作用外,(+)-α-蒎烯、β-蒎烯、(+)-3-蒈烯和异松油烯对横坑切梢小蠹均表现出了明显的引诱作用;行为反应多重比较表明,(+)-α-蒎烯在10 g·L-1浓度下对雌雄虫的引诱作用最大。该研究结果可为开发横坑切梢小蠹林间引诱剂提供参考。
 

关键词: 生源要素, 养分回收, 生物地球化学循环, 土壤类型, 氮磷平衡

Abstract:

In order to screen out the effective compounds for preparation of attractants to trap female and male Tomicus minor (Hartig), the electroantennogram (EAG) and behavioral responses of female and male adults of T. minor to volatiles of its plant host, Pinus yunnanensis Franchet were tested. The results showed that all the 9 tested plant volatiles could induce the EAG responses at 10 g·L-1 compared with the control, and the EAG responses of the antennas caused by the different volatiles had significant differences (P<0.05). The female and male adults of T. minor exhibited strong EAG responses to (+)-α-pinene, (+)-limonene, (+)-3-carene, β-pinene and terpinolene; In the dose response tests, the EAG responses of female and male adults to (+)-α-pinene, (+)-limonene, (+)-3-carene and β-pinene intensified with the increasing concentration. When the concentration increased to 10 g·L-1, the EAG response was the strongest; however, the females and males showed the strongest EAG responses to terpinolene at the concentration of 1 g·L-1. Furthermore, T. minor showed no distinct directional behavior to the (+)-limonene; however, (+)-α-pinene, β-pinene, (+)-3-carene and terpinolene could obviously allure the females and males. The Duncan’s multiple range test for the behavioral response of females and males to the five plant volatiles showed that (+)-αpinene had the greatest attracting effect on females and males at 10 g·L-1 concentration. Our study provides a reference for developing attractive chemicals for T. minor in forests.
 

Key words: biogenic element, nutrient resorption, N and P balance, biogeochemical cycle., soil type