耐盐解烃菌的分离鉴定及其对石油污染盐碱土壤生物修复

生态学杂志

耐盐解烃菌的分离鉴定及其对石油污染盐碱土壤生物修复

王君^1,2,3**,范延辉^1,2,吴涛^1,2,谢文军^1,3,姚志刚^1,2,3,张韩杰^1,2

(¹滨州学院生命科学系，山东滨州 256603； ²山东省黄河三角洲野生植物资源开发利用工程技术研究中心，山东滨州 256603； ³黄河三角洲生态环境研究中心，山东省黄河三角洲生态环境重点实验室，山东滨州 256603)

出版日期:2013-12-10 发布日期:2013-12-10

Isolation and identification of salt-tolerant hydrocarbondegrading Rhodococcus strain and its roles in bioremediation of petroleum-contaminated saline soil.

WANG Jun^1,2,3**, FAN Yan-hui^1,2, WU Tao^1,2, XIE Wen-jun^1,3, YAO Zhi-gang^1,2,3, ZHANG Han-jie^1,2

(¹Department of Life Sciences, Binzhou University, Binzhou 256603, Shandong, China; ²Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, Binzhou 256603, Shandong, China; ³Research Center for EcoEnvironmental Sciences Yellow River Delta, Shandong Key Laboratory of EcoEnvironmental Science for Yellow River Delta, Binzhou 256603, Shandong, China)

Online:2013-12-10 Published:2013-12-10

摘要/Abstract

摘要：

从黄河三角洲石油污染盐碱土壤中筛选到1株产生物乳化剂的耐盐解烃菌JK-7，初步鉴定该菌属于红球菌属，命名为Rhodococcus sp. JK-7。对该菌的耐盐解烃特性以及耐盐产生物乳化剂的特性进行了研究，在实验室模拟了菌株JK-7对石油污染盐碱土的生物修复情况。该菌能够在含有0～12% NaCl的培养基中以石油烃为唯一碳源生长，对原油的降解率达74.5%。以柴油为碳源，在0～5%的最适NaCl浓度范围内，可产生一种生物乳化剂；该生物乳化剂对柴油具有很好的乳化效果，且具有耐高温、耐盐碱的特性。室内模拟实验结果表明，菌株JK-7产生的生物乳化剂能够明显促进菌株对土壤中原油的降解，在石油污染盐碱土壤的生物修复中具有很好的应用前景。

Abstract: A salt-tolerant hydrocarbon-degrading strain which can produce bioemulsifer was isolated from a petroleumcontaminated saline soil in the Yellow River Delta of China. This strain was preliminarily identified to be belonged the genus Rhodococcus, and named as Rhodococcus sp. JK-7. The characteristics of the hydrocarbondegradation and of the produced bioemulsifer of the strain under high concentration NaCl were studied under laboratory conditions, and the bioremediation of the petroleumcontaminated saline soil by the strain JK-7 were simulated. The strain JK-7 could grow with hydrocarbon as the sole carbon source under the NaCl concentration of 0-12%, and the degradation rate of crude oil by the strain reached 74.5%. This strain could produce a bioemulsifer with diesel as the sole carbon source under the NaCl concentration of 0-5%, and this bioemulsifier was characterized by good emiulsification effect, high thermostability, and high resistance to alkali and salt. The simulation results indicated the bioemulsifier produced by the strain JK-7 could obviously promote the strain to degrade crude oil in saline soil. Therefore, the strain JK-7 could have good prospect in the bioremediation of petroleumcontaminated saline soil.

王君1,2,3**,范延辉1,2,吴涛1,2,谢文军1,3,姚志刚1,2,3,张韩杰1,2. 耐盐解烃菌的分离鉴定及其对石油污染盐碱土壤生物修复[J]. 生态学杂志.

WANG Jun1,2,3**, FAN Yan-hui1,2, WU Tao1,2, XIE Wen-jun1,3, YAO Zhi-gang1,2,3, ZHANG Han-jie1,2. Isolation and identification of salt-tolerant hydrocarbondegrading Rhodococcus strain and its roles in bioremediation of petroleum-contaminated saline soil.[J]. cje.