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生态学杂志 ›› 2010, Vol. 29 ›› Issue (07): 1371-1376.

• 研究报告 • 上一篇    下一篇

养殖密度对银鲳幼鱼生长及组织生化指标的影响

彭士明,施兆鸿**,孙 鹏,尹 飞,王建钢   

  1. 中国水产科学研究院东海水产研究所,上海 200090
  • 出版日期:2010-07-08 发布日期:2010-07-08

Effects of breeding density on the growth and tissues biochemical indices of juvenile silver pomfret (Pampus argenteus).

PENG Shi-ming, SHI Zhao-hong, SUN Peng, YIN Fei, WANG Jian-gang   

  1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
  • Online:2010-07-08 Published:2010-07-08

摘要: 养殖密度是水产养殖过程中不容忽视的关键因子之一,可直接影响到养殖成活率、生长性能、游动行为、健康状况、水质、摄食情况以及养殖产量。较高的养殖密度导致水质恶化、过度拥挤以及不利的群体效应等,从而会引起一系列的应激反应,最终影响到养殖效果。银鲳作为一种新兴的养殖品种,目前国内尚未见有关其养殖生物学方面的研究报道。本文的目的是研究养殖密度对银鲳(Pampus argenteus)幼鱼(5.33±0.07)g生长、组织中糖元与乳酸含量以及超氧化物歧化酶(SOD)、过氧化氢(CAT)和谷胱甘肽过氧化物酶(GSH-PX)活性的影响。本试验共设4组放养密度,分别为5、10、15、25尾·m-3,依次编为D1组、D2组、D3组、D4组,试验周期为60 d。结果显示,在低于15尾·m-3(D3组)的密度范围内,银鲳的生长与养殖密度呈正相关,但在D4组,银鲳的生长率相对于D3组明显降低;各处理组间肝体指数与成活率并无显著性差异;随着养殖密度的提高,肌肉与肝脏中糖元含量明显降低,但肌肉乳酸含量并未有显著性变化;在D1至D3组密度范围内,养殖密度对肌肉与肝脏中SOD与CAT活性均未有显著性影响,但D4组的SOD活性均显著低于D2和D3组,且D4组肝脏CAT活性显著性低于D3组;然而,养殖度对肌肉CAT活性以及组织中GSH-PX活性并未造成显著的影响。在试验周期内,本试验所设的养殖密度范围对银鲳并未产生明显的不良胁迫反应。

关键词: 林窗, 林窗边缘, 热力效应, 水湿效应, 时空分布

Abstract: Breeding density is one of the most important variables in aquaculture, because it directly affects the survival, growth, behavior, health, feeding, and productivity of aquatics and the water quality. Breeding at high density can cause the deterioration of water quality, overcrowding of aquatics, and adverse colony effect. Silver pomfret (Pampus argenteus) is a new candidate species for aquaculture. Until now, few studies have been conducted on the breeding biology of P. argenteus. The aim of this study was to evaluate the effects of breeding density on the growth of juvenile P. argenteus (5.33±0.07 g), and the glycogen and lactate contents as well as the superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX) activities in the liver and muscle of the juveniles. The juveniles were kept at the breeding densities of 5, 10, 15, and 25 individuals·m-3(named D1, D2, D3 and D4, respectively) for 60 days. At the density up to D3, the growth rate of the juveniles increased; whereas at D4, the growth rate decreased. No significant difference was found in the hepatopancreas index and survival rate among the treatments. The glycogen content in the liver and muscle of the juveniles was negatively correlated with breeding density, being the lowest at D4, while the lactate content in the muscle had no significant correlation with the breeding density. At the densities from D1 to D3, the activities of SOD and CAT in the liver and muscle had less change; but at D4, the SOD activity in the liver and muscle was significantly lower than that at D2 and D3, and the CAT activity in the liver was significantly lower than that at D3. No significant differences in the activities of muscle CAT and liver-and muscle GSH-PX were observed among all treatments. In general, the test breeding densities were not very stressful to the juvenile P. argenteus during the whole experimental period.

Key words: Canopy gap, Edge of canopy gap, Thermal effect, Water vapor effect, Temporal spatial distribution