欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志

• 研究报告 • 上一篇    下一篇

铜污染对天蓝苜蓿幼苗生长及活性氧代谢的影响

储玲1,2;晋松1,2;吴学峰1,2;刘登义1,2   

  1. 1安徽师范大学重要生物资源保护与利用研究安徽省重点实验室, 芜湖 241000;
    2安徽师范大学生命科学学院生物多样性研究中心, 芜湖 241000
  • 收稿日期:2005-12-28 修回日期:2006-09-20 出版日期:2006-12-10 发布日期:2006-12-10

Effects of Cu pollution on Medicago lupulina L. seedlings growth and active oxygen metabolism

CHU Ling1,2;JIN Song1,2;WU Xuefeng1,2;LIU Dengyi1,2   

  1. 1Provincial Key Laboratory of Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu 241000, China;
    2Biodiversity Research Center, Anhui Normal University, Wuhu 241000, China
  • Received:2005-12-28 Revised:2006-09-20 Online:2006-12-10 Published:2006-12-10

摘要: 通过盆栽实验研究了重金属铜(Cu)污染对天蓝苜蓿 (Medicago lupulina L.) 幼苗的生长及活性氧代谢系统的影响。结果表明,低Cu污染(<500 mg·kg-1)对天蓝苜蓿 幼苗生长无明显抑制现象,甚至还具有一定的促进作用,电导率略微升高,而植株鲜重、干 重、叶片可溶性蛋白质含量及叶片色素含量均在500 mg·kg-1处理浓度时达到峰值。 同时,丙二醛(MDA)水平降低,活性氧清除系统内超氧化物歧化酶(SOD)、过氧化物酶( POD)、过氧化氢酶(CAT)活性均略微升高,保护酶系统仍保持平衡。但随Cu浓度继续增加 (500—3 000 mg·kg-1)则显示对幼苗生长的一定的负效应,与对照组相比,植株 鲜重、干重和可溶性蛋白质含量均显著下降,叶片电导率明显增大,MDA水平上升,且SOD和 CAT活性显著下降(分别下降了19.14%和20.81%),而POD活性却明显上升(比对照上升了2.01倍),表明活性氧清除系统遭到破坏,保护酶系统失衡。

关键词: 栽培方式, 籼稻, 粳稻, 根表铁膜, 镉污染土壤

Abstract: The study with pot experiment showed that low concentrations of Cu (<500 mg·kg -1) had no obvious inhibitory effects on the seedlings growth of Medicago lupulina L., while in adverse, could promote it. The leaf electric conductivity increased slightly, and the plant fresh and dry weights as well as the leaf soluble protein and pigments contents all reached their highest values at 500 mg Cu·kg-1.In the meantime, leaf MDA content decreased,superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities slightly increased, and protective enzy me system still kept in balance. However, definite negative effects of Cu were o bserved with the increase of Cu concentration (500—3000 mg·kg-1). Compared with the control,the plant fresh and dry weights as well as the leaf soluble protein and pigments contents decreased drastically, and leaf electric conductivity and malondialdehyde (MDA) content increased rapidly. Meanwhile, the POD activity increased obviously while SOD and CAT activities decreased markedly, which indicated that the active oxygen metabolism system was destroyed, and the balance of protective enzyme system was broken.

Key words: Cultivation practices, Indica, Japonica, Iron plaque outside roots, Cd-polluted soil