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    10 March 2015, Volume 34 Issue 3
    Stand layer index and its relations with species diversity of understory shrubs of Cunninghamia lanceolata plantations.
    CAO Xiao-yu, LI Ji-ping**, ZHOU Yong-qi, DENG Chao
    2015, 34(3):  589-595. 
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    The variation characteristics of stand layer index and the species diversity of understory shrubs in three age groups (juvenile, medium and near mature) of Cunninghamia lanceolata stands and their correlations were analyzed based on the observation of C. lanceolata stands in eighteen permanent plots in Fushou Forest Farm of Hunan Province. The results showed that there were significant differences in stand layer index among different age groups (P<0.05). The stand layer index of the three age groups ranged from 0.134 to 0.507, indicating that stand layer index was generally low, the tree’s vertical space was underutilized, and the differentiation between individuals was weak. As C. lanceolata trees grew, species richness, Simpson diversity index and Shannon index of understory shrubs increased first and then decreased. There were significant differences in richness between the juvenile and medium stands (P<0.05), while the Simpson evenness and Pielou index of understory shrubs varied insignificantly with the increase of tree age. The species richness index had a very significant positive correlation with the stand layer index, indicating that stand layer index was an important factor influencing the richness index of understory shrubs of C. lanceolata stands.
     
    Effects of intraspecific competition and litter coverage on fine root morphological traits of Cunninghamia lanceolata and Loropetalum chinensis.
    WANG Jun-long1,2, WANG Hui-min1, FU Xiao-li1**, YANG Feng-ting1, CHEN Fu-sheng3
    2015, 34(3):  596-603. 
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    Fine roots play a key role in soil nutrient cycling. Most studies of fine root plasticity have focused on tree species in forest ecosystems, however, there is limited understanding of fine root plasticity of understory species. In order to understand the effects of intraspecific competition and forest management measure (litter covering) on fine root morphological traits and nutrient uptake strategy, a rootbag method was employed to investigate the effects of intraspecific competition and litter coverage on fine root morphological traits (specific root length, diameter, and branch ratio) of Cunninghamia lanceolata (tree species) and Loropetalum chinensis (shrub species). We found that litter coverage and competition had no significant influence on fine root morphological traits of C. lanceolata. However, litter coverage significantly improved the first two orders fine root branching ratios of L. chinensis. Litter coverage, intraspecific competition and their interaction effect significantly decreased the diameters of 1 and 2order fine roots of L. chinensis. These changes of L. chinensis fine root traits might increase the fine root nutrient absorption rate. Our results indicated that litter addition to C. lanceolata forest might help to improve the nutrient absorption ability of L. chinensis by influencing its fine root morphological traits and thus would enhance the nutrient competition between the overstory and understory species at the same soil depth.
    Effects of light intensity on growth and photosynthesis of three karst plant seedlings.
    WANG Man-lian, WEI Xiao, TANG Hui, LIANG Hui-ling**, ZOU Rong
    2015, 34(3):  604-610. 
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    In order to explore the effects of light intensity on the growth and photosynthesis of species distributed in the karst area, the morphology, biomass allocation and photosynthesis physiological characteristics of Ardisia corymbifera, Illicicum difengpi and Pittosporum pulchrum two-year-old seedlings were compared under 100%, 50% and 25% light levels. The results showed that shading environment facilitated the height, total leaf area and crown area of the three plant seedlings. Overall, the root mass fraction and root mass/crown mass ratio of the three species increased significantly, and leaf mass fraction, leaf area ratio and specific leaf area decreased significantly with the increase of light intensity. The net photosynthetic rate (Pn) of A. corymbifera and I. difengpi was significantly lower under full light than under shading environment, implying that stomatal restriction was the main factor leading to the decline of Pn. The Pn of P. pulchrum increased significantly with the increase of light intensity. With the increase of light, chlorophyll content per unit area of I. difengpi and P. pulchrum decreased significantly, while the carotenoid content per unit area, chlorophyll a/b and carotenoid/chlorophyll ratios of A. corymbifera increased significantly. The total biomass of A. corymbifera and I. difengpi seedlings reached the maxima under 50% shading, and the total biomass of P. pulchrum significantly increased with the increase of light intensity. These three karst plant seedlings adapted to different light conditions by different plant morphology and characteristics of photosynthesis. P. pulchrum preferred to strong light, while the seedlings of A. corymbifera and I. difengpi inclined to the shading environment.
    Vegetation landscape change and its multi-scale response relationship with terrain in Loess Plateau. 
    ZHANG Jian-xiang1,2, ZHANG Duo-yong3**, ZHANG Bo4, ZHAO Yi-fei5, LIU Wan-feng1,2
    2015, 34(3):  611-620. 
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    Based on remote sensing (RS) and geographic information system (GIS) technology, and using the normalized difference vegetation index (NDVI) of different resolution in the Loess Plateau from 1982 to 2011, the multi-scale change characteristics of different periods of the Loess Plateau vegetation landscape in relation to the terrain were analyzed by multi-scale spatial statistics method of wavelet analysis. The results indicated that the vegetation landscape of Loess Plateau exhibited periodic changes from 1982 to 2011, mainly including three phases, namely the vegetation restoration phase before 1990, the vegetation degradation phase from 1990-2001 and vegetation recovery phase after 2001. Furthermore, the spatial heterogeneity, data acquisition time, data resolution and spatial position of vegetation landscape had good correlations with topography in the Loess Plateau. On one hand, higher vegetation index and data resolution caused greater spatial heterogeneity of vegetation landscape, and the spatial heterogeneity of vegetation landscape along the longitude direction was greater than that along the latitude direction. On the other hand, the multi-scale NDVI variation was correlated to topographical factors, and the correlation coefficients ranked as follows: DEM > CTI > slope > aspect. These results will be of great significance for vegetation landscape rational adjustments, biodiversity enhancement, soil erosion control, and landscape continuity enhancement, and provide important basis for ecological environment construction in the Loess Plateau.
    Habitat suitability assessment and conservation strategies for giant pandas in Fengtongzhai Nature Reserve, China.
    RONG Zhan-lei, ZHANG Jin-dong, HONG Ming-sheng, YUAN Shi-bin, ZHANG Ze-jun**
    2015, 34(3):  621-625. 
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    Habitat quality evaluation is the basis for making scientific conservation measures and enhancing protection efficacy for the endangered wildlife. Fengtongzhai Nature Reserve is the type locality of giant panda (Ailuropoda melanoleuca), which is located in the middle of Qionglai Mountains. Previous studies associating with giant panda protection focused on habitat selection, while habitat suitability studies have not yet reported. In order to promote the scientific conservation of the wild giant panda population, we evaluated the habitat suitability of the giant panda in the reserve using landscape connection degree model. Four ecological factors including altitude, slope, vegetation type and food sources were selected as the indicators. The result showed that the unsuitable habitat area, suitable habitat area and the most suitable habitat area were 223.26, 133.67 and 33.46 km2, accounting for 57%, 34% and 9% of the total area of the reserve, respectively. The spatial distribution of the most suitable habitat and the suitable habitat were relatively scattered in the reserve. The sign locations of giant pandas were mainly found in the higher degree suitable habitat regions. We suggest those that sensitive areas with human activities should be emphasized in future management for ensuring the dispersal of individuals among different habitat patches. Especially, the daily routine work of protection and management should focus on the region connected to Huangshuihe Nature Reserve, and the management extent of Fengtongzhai Nature Reserve should be expanded to some degree.
    Nitrogen sensitivity of four epiphyte lichens from habitats of Rhinopithecus roxellana in Shennongjia Nature Reserve.
    WANG Chuan-hua1,2**, YANG Lin1, YUAN Qiao-ling1, Silvana Munzi3, LIU Ming-jun4
    2015, 34(3):  626-633. 
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    To explore the potential impacts of nitrogen deposition on lichen diversity in SnubNosed Monkey (Rhinopithecus roxellana) habitats in Shennongjia, an insitu experiment was conducted in a forest in Shennongjia Nature Reserve. Four common epiphytic lichens (Usnea longissima, Usnea betulina, Ramalina calicaris var. japonica and Parmotrema hypoleucinum) were collected, then soaked in solutions of KNO3, NH4NO3, (NH4)2SO4, or deionized water three times, for half an hour each time at 0, 24 and 48 h, respectively; then Fv/Fm, cell integrity (measured as solution electrical conductivity, RE) and chlorophyll OD435/OD415 were measured after 72 h, which are indexes indicating lichen sensitivity to nitrogen deposition. The results showed that higher nitrogen concentration led to a significant decrease of Fv/Fm and OD435/OD415 and a significant increase of RE. Furthermore, speciesspecific differences in sensitivity were found for different nitrogen types and different indications. Based on the response of Fv/Fm, U. longissima and U. betulina were more sensitive to KNO3 than to NH4SO4 and NH4NO3, but R. calicaris var. japonica and P. hypoleucinum were more sensitive to (NH4)2SO4 than to KNO3 and NH4NO3. According to RE response to nitrogen treatment, U. longissim, U. betulina and P. hypoleucinum were most sensitive to (NH4)2SO4, but there was no significant difference among responses of R. calicaris var. japonica to KNO3, NH4NO3 and (NH4)2SO4. As with OD435/OD415, U. longissim and P. hypoleucinum were more sensitive to (NH4)2SO4 than to KNO3 and NH4NO3, U. betulina was more sensitive to KNO3 and (NH4)2SO4 than to NH4NO3, but R. calicaris var. japonica was equally sensitive to the three nitrogen types. The results suggested that more attention should be paid to negative effects of nitrate deposition on habitats of SnubNosed Monkey at Shennongjia Nature Reserve, and further investigation on effects of nitrogen deposition on lichen biomass accumulation and nutrition components should be conducted to a perfect conservation of Sichuan SnubNosed Monkey.
    Niche characteristics of dominant tree species in Macaca mulatta habitat of Lishan National Nature Reserve, Shanxi. 
    TIE Jun1,2**, LI Yan-fen3, JIN Shan1,2, CHEN Yan-bin1, ZHANG Gui-ping1,2, RU Wen-ming1,2
    2015, 34(3):  634-641. 
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    The niche characteristics of 22 dominant species, including 12 trees and 10 shrubs, in Macaca mulatta habitat of Lishan National Nature Reserve were analyzed by using Shannon’s niche breadth and Levins’s niche overlap indices. The results indicated that: (1) Carpinus turczaninowii had the largest niche breadth (3.477), followed by Carpinus cordata (3.340), Juglans cathayensis (3.194), Quercus mongolica (3.123), and Ulmus pumila had the least niche breadth (1.039) in the tree layer. In the shrub layer, the niche breadth value of Forsythia suspensa was the largest (3.491), followed by Spiraea trilobata (3.017), Sorbaria sorbifolia (1.938), Elaeagnus pungens (1.759), Lonicera ferdinandii (1.593), and Deutzia grandiflora had the least (1.131). The results suggested that these shrubs had strong adaptability to the environment and competition for the resources. (2) There were low niche overlaps among 22 trees. The niche similarity coefficients were from 0.10 to 0.30, suggesting that interspecific competition among those trees was relatively weak. (3) There was no complete positive correlation between the niche breadth and niche overlap, and this result may be determined by the heterogeneity of the habitat.
    (Puer College, Puer 665000, Yunnan, China)
    CUI Xian-liang, LUO Ya-ting, SU Zhi-long, LUO Yin-ling**
    2015, 34(3):  642-647. 
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    The effects of light condition (12 h photoperiod and 24 h dark) on the seed germination of differentaltitude Spiraea rosthornii (2500, 2700, 3020, and 3300 m) and Berberis aggregata (2160, 2760, 3000, and 3250 m) from the eastern QinghaiTibet plateau were studied. The results are as follows: (1) The seed size of S. rosthornii was significantly negatively correlated with altitude, and the seed size of B. aggregata had no significant correlation with altitude. (2) The germination rates of S. rosthornii seeds under light or dark and germination percentage under dark hada significant positive correlation with altitude, but a weak positive correlation was found for germination percentage under light. The germination rate and germination percentage of B. aggregata seeds under light or dark condition had no correlation with altitude, but the germination rate or germination percentage had significant difference among the seeds from different altitudes. The B. aggregata seeds from 2760 m altitude had the highest germination ability, the higher or lower altitudes both led to lower germination. (3) The seeds of S. rosthornii from different altitudes had significantly higher germination rates and germination percentages under light than under dark, but the difference was not significant when the seeds were from 3250 m altitude. The light condition had no effects on seed germination rate and germination percentage of B. aggregata from different altitudes. These results indicated that the altitude and light condition were the main reasons that led to lower germination ability of S. rosthornii seeds. For B. aggregata, the seeds from different altitudes had different germination abilities, but light condition had no effects on the germination.
    Allometric relationship between modular morphology and biomass of four annuals in the Gurbantunggut Desert, China.
    XIE Ran1, TAO Ye2**, CHANG Shun-li1
    2015, 34(3):  648-655. 
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    Annuals are an important component of herbaceous layer in summer and autumn in the Gurbantunggut Desert, China. In this study, four annuals (Salsola ruthenica Iljin, Agriophyllum squarrosum L. Moq., Ceratocarpus arenarius L., and Horaninowia ulicina Fisch. et Mey.) in Chenopodiaceae were chosen as targets, and the modular morphology, biomass allocation and their allometric relationships were comparatively analyzed. The results indicated that A. squarrosum had the biggest body size (volume and biomass), followed by S. ruthenica, and C. arenarius and S. pycnophylla were the smallest. S. pycnophylla had the highest ratio of root to shoot biomass (R/S), followed by S. ruthenica, and A. squarrosum and C. arenarius were the lowest. There were significant positive correlations between modular morphology and biomass, indicating a strong tendency of collaborative change. R/S showed negative correlations between most morphological variables and biomass, indicating that the allocation ratio of belowground biomass decreased gradually with the increase of plant size. Most of the modular morphology and biomass indices represented significant allometric relationships with the scaling exponent <1.0, but most of the allometric scaling exponents among the four annuals were not different and these species all had the common scaling exponent each. This result revealed that although the plant sizes of the four species varied, the four annuals had the same growth ratio and collaborative change characteristics among these modular morphology and biomass indices, reflecting the convergent adaptation of different species in the lifeform ‘annuals’ to arid deserts.
    Effect of intercropping sugarcane with peanut on weed seed germination in sugarcane soil.
    SHEN Xue-feng, FANG Yue, DONG Zhao-xia, CHEN Yong**
    2015, 34(3):  656-660. 
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    A field study was conducted to evaluate the effects of intercropping on weed seed germination in sugarcane soil. Four treatments were designed: natural recovery, peanut monoculture, sugarcane monoculture, sugarcane intercropping with peanut. The results showed that the weed seed density and species in the soil of sugarcane intercropping with peanut were reduced by 44.4% and 34.0% compared with peanut monoculture, and by 37.5% and 22.7% compared with sugarcane monoculture, respectively. Compared with soil seed bank before sowing soil and in the natural recovery plots, the dominant species of weed increased but their seed density decreased significantly in the soil of intercropping sugarcane with peanut. The weed yield of intercropping sugarcane with peanut accounted for 33.7%, 40.9% and 55.8% of those in natural recovery, peanut monoculture, and sugarcane monoculture, respectively. Our study suggests that intercropping affects the density and species composition of the weed seedbank by producing higher crop yield and less weed growth through usurping resources from weeds and by suppressing weed growth through allelopathy.
    Effects of tuber-feeding waterbird guild and water level fluctuation on tuber distribution of submerged macrophytes in Shahu Lake.
    LI Lei1,2, ZHANG Xiao-chen1, QIN Hai-ming1, HU Xu-ren1, CHEN Jia-kuan1,2**
    2015, 34(3):  661-669. 
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    The variations in tuber distribution in a dishshaped sublake of Poyang Lake and the consumption by tuberfeeding waterbirds were investigated in two years with different water level conditions. Our aims were to explore impacts of wintering waterbird foraging and water level fluctuation on tuber distribution of submerged macrophytes. Four investigations on species, density and biomass of tubers of submerged plants were conducted in Shahu Lake in October 2012, April 2013, October 2013 and April 2014, respectively, using quadrat method. The results showed that there were two different kinds of tubers occurring in the same distribution area in Shahu Lake, which derived from Vallisneria spinulosa and Hydrilla verticillata var. rosburghii, respectively. Both their density and biomass were significantly lower in October 2013 than in October 2012. This is mainly due to the dramatic interannual changes in water level of Poyang Lake during flood seasons and resultant significant impact on water quality. It was found that both the duration in which Shahu Lake was connected to the main region of Poyang Lake and the average daily water depth during April to September (flood season) in 2013 were significantly lower than in 2012, while the water turbidity during that period in 2013 was much higher. This condition was not advantageous to the growth and development of submerged plants. The density and biomass of the two kinds of tubers declined significantly at the end of wintering period of 2012-2013, while both of them remained unchanged before and after the wintering period of 2013-2014. The foraging activities of tuberfeeding waterbirds and the negative effects of high water level on food
    availability could probably account for these differences. In short, the dramatic hydrological fluctuation in Poyang Lake would result in interannual fluctuation in food resources of waterbirds, while the food availability would be influenced by both tuber abundance and its accessibility by tuber feeders during the wintering period. This study is of important guiding significance to submerged macrophytes revegetation, waterbird conservation, as well as ecosystem function estimation for Poyang Lake and even for regions along the middle and lower reaches of the Yangtze River.
    Estimation of ecological water requirement of desert vegetation in the arid region of Northwest China.
    ZHOU Dan1,2, SHEN Yan-jun2**, CHEN Ya-ning3, GUO Ying2, ZHANG Bo1
    2015, 34(3):  670-680. 
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    In this paper, we calculated the fractional vegetation cover (fc) within growing season in the arid region of Northwest China based on two kinds of NDVI data (AVHRR-GIMMS and SPOT-VEGETATION). The desert vegetation area was determined according to the thresholds of vegetation cover, and which was validated in the Tarim River Basin. The ecological water requirement of desert vegetation in the arid region of Northwest China was calculated using the phreatic evaporation model with the area of desert vegetation and the reference crop evapotranspiration (ET0) during 1982-2010. The results were expected to provide evidence for water use management in this area in future. Results showed that the thresholds of fc were reasonable to be 0.1-0.35 to determine the desert vegetation area in this area. There was no drastic change in area of desert vegetation during 1982-2010, which showed an increasedecreaseincrease trend. The mean areas of high vegetation cover and low vegetation cover were 2.343 million and 4.482 million hm2, respectively. The annual ET0 in the Tarim River Basin was higher than that in Northern Xinjiang, and the ET0 in eastern part was larger than that in western part. The mean annual ET0 values in Tarim River Basin, Northern Xinjiang and QilianHexi area were 1066.9, 975.6, and 1171.6 mm, respectively. Phreatic evaporation of the desert vegetation in the arid region of Northwest China was calculated according to the data fitting of the ET0. In the Tarim River Basin, Northern Xinjiang and QilianHexi area, the mean annual phreatic evaporation values of the lowcover desert vegetation were 75, 64 and 82 mm, respectively; the mean annual phreatic evaporation values of the highcover desert vegetation were 275, and 233 and 299 mm, respectively. The mean annual ecological water requirement of desert vegetation was 13.413 billion m3 in the arid region of Northwest China, with a reduced rate of 10.63 million m3·a-1 during 1982-2010. In area with high vegetation cover, the mean annual ecological water requirement was 9.164 billion m3, with a reduced rate of 30.34 million m3·a-1, while that in area with low vegetation cover was 4.249 billion m3, with an increased rate of 19.72 million m3·a-1.
    Effects of saline-sodic stress on organic acid in sweet sorghum.
    DAI Ling-yan1, TANG Cheng-rui1, YIN Kui-de1, RUAN Yan-ye2, ZHANG Li-jun2**
    2015, 34(3):  681-687. 
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    In order to understand the effects of salinesodic (based on the composition of Na2CO3 and NaHCO3) stress on the organic acid contents in sweet sorghum plant (Sorghum dochna (Forssk.)), the changes of organic acid contents and phosphoenolpyruvate carboxylase (PEPC) activities in above and underground parts of sorghum varieties 314B and M-81E were studied. The results showed that eight different acids (i.e., oxalic acid, tartaric acid, formic acid, pyruvic acid, malic acid, lactic acid, acetic acid, and citric acid) were detected from both of the above and underground parts, while succinic acid was only found in the roots under salinesodic stress. Meanwhile, the levels of most organic acids decreased with the increment of stress level, whereas few of them increased slightly. The PEPC activity increased at low salinesodic concentration, but decreased at high salinesodic concentration. There was no correlation between organic acid level and PEPC activity. Nevertheless, when the PEPC activity increased, the decreasing rate of organic acids changed slightly and vice versa. In terms of the levels of organic acid and PEPC activity, M-81E (stronger tolerance) suffered a weaker damage than 314B (lower tolerance) under salinesodic stress.
     
    Relationship of yield components and temperature and light factors for super double-cropping late rice.
    NING Jin-hua1,3, LU Kui-dong2,3**, SONG Zhong-hua1, ZHANG Yan-gui1, ZHOU Yu1,3
    2015, 34(3):  688-694. 
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    Using the data of super rice Yueyou 6135 with different sowing dates in 2011-2013, the differences of yield components between different years and sowing dates were analyzed, and the relationship models between yield components and temperature, light and their combination were established based on daily temperature and sunshine data during yield formation. The results showed that the yield component of rice with later sowing date in the same year was relatively poor, and fluctuated between different years. As the sowing date delayed, the differences in yield component of rice at the same sowing date became greater among different years. The best sowing dates were from midJune to early July. Accumulated temperature showed a down parabola relationship with grain number per spike, and when the accumulated temperature reached 873 ℃, the grain number per spike was the highest. The accumulated temperature and sunshine had convex down relationships with empty grain rate, and had convex up relationships with seed setting rate. When 700-920 ℃ accumulated temperature and ≥180 h sunshine were simultaneously met, super rice had higher seed setting rate and lower empty grain rate. The results of this study had important guiding significance to arrange sowing date and utilize climate resources rationally in regions of the middle and lower reaches of the Yangtze River.
     
    Effects of water depth change on energy distribution in paddy rice field.
    LIU Bin1,2, HU Ji-chao1,2**, YU Li3
    2015, 34(3):  695-702. 
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    The variations of water depth and wetdry alternation in paddy field lead to the energy budget changing vertically. In this paper, an investigation was made to quantify the contribution of water depth, leaf area index and meteorological factors on energy distribution by using multiple regressions. Similar meteorological conditions and leaf area index, which can reduce the differences of energy input and canopy structure respectively, were chosen to quantify the effects of water depth on energy partitioning in a paddy field. The results showed that, with the increase of water depth, both water heat storage and water heat flux above soil layer increased, leading to a decrease in the latent heat during the daytime, especially in the period with sparse canopy. However, due to the decrease in net radiation of water surface in the period with dense canopy, the effects of water depth on the latent heat flux became small. The effect of water depth on sensible heat was not obvious, and could be ignored. The simulated results of the dualsource evapotranspiration model showed that the decrease of water latent heat with the increase of water depth was a major cause reducing the total latent heat, and this phenomenon was more obvious in the period with sparse canopy.
    Long-term prediction models for China national wheat powdery mildew based on large scale factors.
    YU Cai-xia1, HUO Zhi-guo1,2**, HUANG Da-peng3, JIANG Yan4, WU Li1
    2015, 34(3):  703-711. 
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    Based on China national wheat powdery mildew occurrence area data from 1971 to 2008, the monthly data of atmospheric circulation characteristics from 1970 to 2008 and the north Pacific sea surface temperature (SST) monthly data from 1970 to 2008, using the method of factor puffing and spatial topological analysis, in combination with atmospheric circulation factors and SST factors, the factors that have stable and significant effects on wheat powdery mildew were selected by means of optimization process, correlation analysis, stability test, and factor independence test, the atmospheric circulation and the north Pacific SST regression forecasting models of the wheat powdery mildew occurrence area rate were constructed, respectively. With data from 2009 to 2010, the models were verified. The accuracy rate of forecast about the atmospheric circulation model with the  historical data reached 81.6% with the predicting accuracy of 100%, and its accuracy rate of prediction was 82.5% as a whole. The accuracy rate of forecast about the north Pacific SST model with the  historical data reached 78.95% with the prediction accuracy of 100%, and its accuracy rate of predicting was 80% as a whole. The evaluation precision of the atmospheric circulation model is slightly higher than that of the north Pacific SST model.
    Dry-hot wind effects on physiology of winter wheat.
    ZHANG Zhi-hong1,2**, CHENG Lin1,2, LI Shu-ling3, PENG Ji-yong2, FANG Wen-song1,2
    2015, 34(3):  712-717. 
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    Weather conditions of dry-hot wind were simulated by artificial control to compare the effects of heavy and light degree of dry-hot wind on winter wheat physiology in field at early and middle grain filling stages. The results showed that the dry-hot wind at middle stage of grain filling had more negative effects than at early stage of grain filling, and the heavygrade dry-hot wind harmed more than the lightgrade one. On the 2nd day after heavy dry-hot wind occurred at middle stage of grain filling, the photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Co) of flag leaf decreased by 19.4% to 36.6%, 44.1% to 58.0%, and 24.3% to 41.7%, respectively. For light dryhot wind, the above index decreased by 9.7% to 20.2%, 28.2% to 34.0%, and 19.9% to 31.8%, respectively. The decreased percentage of SPAD and root flux activity (Gn) also reached the maximum with the heavygrade dry-hot wind at middle stage of grain filling. There was no obvious stressed effect on winter wheat for lightgrade dry-hot wind at early stage of grain filling. At early stage of grain filling, Pn and SPAD of flag leaf could repair to a certain extent after dryhot wind weather, but the damaged physiological indexes could not be repaired when the dryhot wind occurred at other stages. Under the condition of Co >0.1 mmol·m-2·s-1, the stressed effects on Tr were more than on Pn.
    Effects of cadmium and Aroclor 1254 on antioxidant enzyme activities and lipid peroxidation levels in Mytilus coruscus.
    PENG Ling1, ZENG Jiang-ning1,2**, HUANG Wei1, CHEN Quan-zhen1, LIU Jing-jing1
    2015, 34(3):  718-726. 
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    The effects of antioxidant enzymes on the digestive diverticula of Mytilus coruscus were studied at different concentrations of cadmium (0.194, 0.388 and 0.775 mg·L-1) and Aroclor 1254 (0.005, 0.01 and 0.05 mg·L-1) under single and combined stress conditions. The results are as follows: (1) Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx) activities changed similarly, being first increased and then inhibited under single and combined stress conditions. The highest dose groups (0.775 mg·L-1 of Cd2+, 0.05 mg·L-1 of Aroclor 1254) reached the peak values of antioxidant defense systems earlier on the first day of single stress. The descending range and rate of antioxidant enzymes activities were positively correlated with the stress time and concentration of stress material. (2) The stress sensitivities of SOD and GSH-Px were higher than that of CAT. (3) The content of malonaldehyde (MDA) of each dose group was significantly increased (P<0.01) as the stress time was prolonged. A positive correlation was found between effect of single stress and stress dose or time. (4) The effect of combined stress was stronger than that of single stress. It could be inferred that oxidative stress in M. coruscus would be produced by exposure to cadmium and polychlorinated biphenyl above ambient levels. The antioxidant defense systems could act as biomarkers to monitor marine multiple contamination of heavy metals and organics.
    Biodiversity of macro grounddwelling arthropods in secondary forests and plantation forests of montane region of eastern Liaoning Province.
    XU Hong-jun1, YU Li-zhong2,3, HUANG Xuan-rui1, ZHU Jiao-jun2,3, YANG Jin-yu1,2**, GAO Shang-lin4, WANG Yong-jun4
    2015, 34(3):  727-735. 
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    Using the pitfall trapping method, the ground-dwelling arthropod communities were investigated in three types of secondary forests (Quercus mongolica, Juglans mandshurica, and broadleaved mixed forest) and two types of plantation forests (Pinus koraiensis and Larix spp.) in a montane region of eastern Liaoning Province during the active time from July to August. A total of 5135 individuals of grounddwelling arthropods were collected, belonging to 7 classes, 18 orders, and 66 groups. Formicidae (20.4%), Silphidae (19.5%), Scarabaeidae (17.2%) and Carabidae (14.5%) were the dominant taxa. The abundance of the functional groups was ranked as saprozoic > omnivore > predator > phytophage. According to the system clustering analysis, P. koraiensis plantation forest, Larix spp. plantation forest and J. mandshurica forest were grouped together; Q. mongolica forest and the broad-leaved mixed forest were clustered into one group. Based on the redundancy analysis (RDA), litter depth, vegetation cover and tree height could explain 85.9% and 81.9% of community distribution variation of ground-dwelling arthropods and beetles, respectively. This study indicated that forest vegetation and litter distribution, and human disturbance in the study region were the key factors influencing the diversity of macro grounddwelling arthropod community.
    PAE coding ethogram in breeding of semifreeranging Amur tiger (Panthera tigris altaica).
    QIAO Zheng-lei1,3**, ZHANG Hong-hai2, MA Jian-zhang3, WEI Qin-guo4
    2015, 34(3):  736-743. 
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    Observations on behaviors of Amur tiger in breeding seasons were made in a semifreeranging area at Taishan Amur Tiger Park from 2006 to 2007 with alloccurrence recording, focalanimal sampling and behavior sampling methods. Behavioral data were collected and coded following the “postureactenvironment” (PAE) coding system that concerns with posture, act, environment and ecological function of behaviors. A total of 14 postures, 47 acts and 98 behaviors were recognized and defined in our study. Based on ecological function of each behavior, all of the recorded behaviors were categorized into 13 groups including foraging, eliminating, thermoregulatory, paring, mating, parental, agonistic, affiliative, aggregation, communication, resting, locomotive and miscellaneous behaviors. The first behavior ethogram of semifreeranging Amur tiger in breeding season was then established following the PAE coding system.
    Characteristics of zooplankton communities off the Feiyun River estuary.
    DONG Kai-xing1,2, XU Zhao-li1,2**
    2015, 34(3):  744-752. 
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    Based on three oceanographic surveys conducted off the Feiyun River estuary (27°46′-27°75′N, 120°66′-121°01′E) in November 2010 (autumn), January 2011 (winter) and May 2011 (spring), the species composition, Shannon diversity, abundance, horizontal distribution, and seasonal variation of zooplankton were investigated, and their relationship to seasonal variation of water masses was also examined. A total of 60 species belonging to 11 groups were identified, including 42 species belonging to 9 groups in autumn, 20 species belonging to 5 groups in winter and 34 species belonging to 8 groups in spring. The abundance of zooplankton was highest in autumn (221.69 ind·m-3), least in winter (19.52 ind·m-3), and intermediate in spring (137.50 ind·m-3). Spatially, the zooplankton was most numerous in the offshore water. The Shannon diversity of zooplankton was highest in autumn followed by spring and winter. The high value area of diversity was situated in the southeast of investigation waters (autumn), in the outer area of Feiyun River estuary (spring) and among archipelago in the north (winter), respectively. In winter and spring, Calanus sinicus was the predominant which determined the distribution pattern of zooplankton abundance. In autumn, Paracalanus aculeatus and Sagitta bedoti exerted the greatest influence on the distribution of zooplankton abundance. Furthermore, the abundance and community composition of zooplankton was influenced by seasonal variation of water masses to some extent.
    Comparison of pelagic and benthic fish assemblages in mussel farming habitat.
    WANG Zhen-hua, LIANG Jin-ling, ZHANG Shou-yu**
    2015, 34(3):  753-759. 
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    Investigations on fish communities at the pelagic and bottom levels of mussel farming habitat were conducted in November of 2013 and February of 2014 respectively, in order to study fish assemblage differences at the two water levels. Sampling stations were located at Gouqi Island of Ma’an Archipelago, Zhejiang Province. Species composition, diversity, relative abundance, biomass and community patterns of fish at the pelagic and benthic waters of mussel farming habitat were analyzed by using diversity indices, relative fishing efficiency indices and multivariate analysis. The results showed that Mugil cephalus and Konosirus punctatus were the dominant species in pelagic waters during autumn and winter, whereas in bottom waters Sebastiscus marmoratus and Larimichthys polyactis were the dominant species during autumn, and Harpadon nehereus and Johnius belangerii were the dominant species during winter. L. polyactis was found with high abundance both in pelagic and benthic waters during both seasons. The relative catch rates in pelagic waters were higher than in benthic waters during both seasons. Higher abundance and biomass of fish were recorded in pelagic waters than in benthic waters during both seasons. The results of multivariate analysis revealed two significantly different fish composition patterns from the pelagic and benthic waters regardless of seasons. It is concluded that the mussel farming habitat can act as a floating artificial reef system. Therefore, we suggest that the floating artificial reefs can be deployed in the design of marine ranching to achieve threedimension conservation effects. Besides, artificial reefs can be set at the bottom of mussel farming area to enhance marine ranching effects.
    Hatching behavior of brownheaded gull Larus brunnicephalus in Hongjiannao of Shaanxi Province, Northwest China.
    WANG Qing-xiong1, XIAO Hong1**, YANG Chao1, LIU Zheng2
    2015, 34(3):  760-764. 
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    By using e-Science information technology, an investigation was made on the hatching behavior of brownheaded gull (Larus brunnicephalus) in the Hongjiannao of Shaanxi Province, Northwest China from May to June, 2014. The diurnal hatching rhythms at different hatching stages were as follows. At daytime, the time of exchanging hatching at the early, medium, and late stages of hatching averaged 7.88±1.08 (n=39), 3.11±0.19 (n=65), and 3.17±0.22 (n=42), the changes of sitting nest direction averaged 27.75±2.04 (n=39), 23.37±0.91 (n=65), and 20.06±1.46 (n=42), and the times of turning eggs over averaged 27.13±2.02 (n=39), 21.11±1.11 (n=65), and 22.39±1.78 (n=42), respectively. At night, the times of exchanging hatching averaged 5.00±0.89 (n=39), 1.32±0.11 (n=65), and 0.95±0.05 (n=42), the changes of sitting nest direction averaged 14.37±1.72 (n=39), 10.95±0.86 (n=65), and 6.62±0.84 (n=42), and the times of turning eggs over averaged 11.25±1.08 (n=39), 8.47±0.77 (n=65), and 5.33±0.55 (n=42), respectively. On the days with strong wind or heavy rain, there existed significant differences in the times of exchanging hatching, the change of sitting nest direction, and the times of turning eggs over at the same hatching stages, as compared with those on fine days. The time intervals of exchanging hatching by the parents were mainly at 04:00—10:00, and 12:00—14:00. At the late stage of hatching, the duration of sitting nest by the parents was as long as (713.29±40.62) min (n=42). It was suggested that the parents controlled the egg temperature via regulating the diurnal hatching rhythm to assure the normal development of egg embryo during daytime and night. At the same time, incubation rhythms of brownheaded gull were compared with those of sympatric relict gull.
     
    Biodiversity of macrobenthic communities in different habitats of Maoyan Island, China.
    LI Hai-hong, JIANG Kuang, BAO Yi-xin**, WANG Hua, REN Peng
    2015, 34(3):  765-772. 
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    From November 2012 to October 2013, macrobenthic assemblages and biodiversities were investigated seasonally in different intertidal habitats of Maoyan Island in Taizhou, Zhejiang Province. The habitats included artificial Kandelia obovata mangrove forests with different ages, Spartina alterniflora marsh, and bare mudflat. A total of 48 species were recorded, belonging to 28 families, 9 classes, and 8 phyla. Arthropods, mollusks, and annelidas accounted for 83.3% of the total. The density characters of quantitative sampling were used to calculate Shannon index, Pielou index, Margalef index, Simpson index, and G-F diversity index. The results showed that the species and density in S. alterniflora habitat were lower than in K. obovata and natural tidal flat habitats. The numbers of species in summer and spring were higher than in autumn and winter. Either Shannon index or G-F index in mangrove forests was higher than in the other habitats. The species diversity in the young mangrove forest was lower than that in the 6 and 8-year-old mangrove forests, but family and genus diversities were higher in the young mangrove forest.  Variance analysis and hierarchical cluster showed that habitat and season were the key factors
    affecting the distribution of macrobenthic communities.
    Allelopathic effect of extracts from Pinus massoniana rhizosphere soil.
    DUAN Jian, YANG Jie**, KANG Jin-lin, YU Chi-fang, TU An-guo
    2015, 34(3):  773-780. 
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    Sparse understory vegetation with single species composition is a main cause of serious soil and water losses in Pinus massoniana forests in red soil region, and it is closely related with allelopathic effects of trees. In order to clarify the influence of allelopathy of tree’s rhizosphere soil on understory vegetation distribution, the effects of rhizosphere soil extracts of Pinus massoniana and Liquidambar formosana on four common herbs, i.e. Cynodon dactylon, Trifolium repens, Paspalum notatum and Poa annua, were studied. The results showed that: (1) Extracts from Pinus massoniana rhizosphere soil had obvious allelopathic inhibition effects on the four receptor plants under 1, 0.5 and 0.25 mg·mL-1 concentrations. L. formosana rhizosphere soil extracts showed inhibition effects under 0.5 and 0.25 mg·mL-1 concentrations, but had a certain stimulation effect under 1 mg·mL-1 concentration. (2) For different concentrations of P. massoniana rhizosphere soil extracts, no obvious allelopathic effects was found on Cynodon dactylon, but the inhibition effects were enhanced with the increase of extract concentrations for the other three herb plants. For the three concentrations of L. formosana rhizosphere soil extracts, C. dactylon presented the effects of promoting at low concentration and repressing at high concentration, while T. repen and P. annua showed the effects of repressing at low concentration and promoting at high concentration, and P. notatum exhibited an inhibition effect with the increase of extract concentration. In terms of allelopathic effects on understory vegetation in P. massoniana pure forest and mixed forest, this study provided a new perspective to explain the understory vegetation status, and improve the vegetation structure and control soil erosion.
    Effects of long-term simulated warming on soil physicochemical properties and plant chemical components of Kobresia humilis meadow.
    YANG Yue-juan1,4, ZHOU Hua-kun1**, YAO Bu-qing1, WANG Wen-ying2, DONG Shi-kui3, YU Xin-chao1,4, ZHAO Xin-quan1, ZHANG Hao5
    2015, 34(3):  781-789. 
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    By employing the longterm simulation temperature experimental test device recommended by the International Tundra Experiment (ITEX), the responses of plant chemical components (crude ash, neutral detergent fiber, acid detergent fiber, lignin, crude fat, crude protein and nitrogen free extract) and soil physicochemical properties (available nitrogen, available potassium, available phosphorus, total nitrogen, total phosphorus, total potassium, organic matter, and moisture content) to warming on degraded and undegraded Kobresia humilis meadows were studied. The results showed that long-term simulated warming significantly affected soil physicochemical properties, and plant chemical components of three functional groups (P<0.05). There were obvious interactive effects between plant chemical components and soil physicochemical properties in degraded K. humilis meadow under long-term simulated warming (P<0.05). The simulated warming increased soil available nutrients which could be directly used by plants, but decreased soil organic matter, total nitrogen, total phosphorus, total potassium and moisture contents in the degraded meadow, compared with the control. Meanwhile, warming decreased the contents of crude fat, crude protein and nitrogen free extract of highquality herbage (Gramineae and Cyperaceae), while increased the contents of some indigestible ingredients such as crude ash, neutral detergent fiber, acid detergent fiber, lignin, consequently, resulting in a decrease in ruminant assimilation ability. Under long-term simulated warming conditions, the contributions of soil environmental factors to plant chemical composition were as follows: available nitrogen > organic matter > moisture content > available potassium. Canonical correspondence analysis showed that longterm warming further increased the advantages of Gramineae and Cyperaceae species in the nondegraded K. humilis meadow, while forbs gradually declined. In the degraded meadow, the dominance of dominant species declined, intensifying the degradation of grassland.
    Effects of different straw incorporation ways on N2O emission and soil physicochemical properties of brown soil.
    2015, 34(3):  790-796. 
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    A field experiment was conducted to investigate the soil physicochemical properties and N2O emissions from brown soil amended with different materials by static chambergas chromatography method. Three treatments, including single fertilization (CK), fertilization and corn stover incorporation (CS) and fertilization and carbonized corn stover incorporation (BC) were set with the same rate of chemical fertilizer. The results showed that: (1) The N2O emissions of BC and CS decreased by 38.9% and 24.0% respectively, compared to CK. (2) Compared with CS and CK, the N2O emission intensity of BC decreased by 35.0% and 39.2% respectively, but no significant difference was observed between CS and CK. (3) Both CS and BC decreased soil bulk density, ammonia oxidation potential, and increased soil pH, available potassium and organic carbon content. (4) Correlation analysis showed that there was a significant positive correlation between N2O emission and soil temperature, soil bulk density or ammonia oxidation potential. A significant negative correlation was found between N2O emission and soil pH or nitrate nitrogen concentration. There was also a negative correlation between N2O emission and soil organic carbon.
    Short-term effect of nitrogen addition on soil respiration of three temperate forests in Dongling Mountain.
    QUAN Quan1,2, ZHANG Zhen1**, HE Nian-peng2, SU Hong-xin3, WEN Xue-fa2, SUN Xiao-min2
    2015, 34(3):  797-804. 
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    To investigate the effect of N deposition on soil respiration of temperate forests, a simulated atmospheric nitrogen (N) deposition experiment was conducted in three typical temperate forests in Dongling Mountain in Beijing. The three forests included oak forest (broadleaf forest), larch forest and pine forest (coniferous forest). In each forest, we set control and N addition treatments (10 g N·m-2·a-1, five times of current atmospheric N deposition) and four replicates (15 m × 15 m). N addition (NH4NO3) and soil respiration measurement were conducted in growing season. The results showed that N addition decreased the soil respiration rate of broadleaf forest but enhanced that in coniferous forest. Under the N enriched condition, the accumulation emission of soil respiration during the growing season decreased by 6.13 g C·m-2 in oak forest while increased by 1.78 g C·m-2 and 5.64 g C·m-2 in larch and pine forests, respectively. However, the shortterm effect of N addition was not significant among three temperate forests. Moreover, soil respiration rate (P<0.001) and the accumulation emission of soil respiration (P<0.001) in growing season among different forests varied significantly in the order of oak forest > pine forest > larch forest. Soil temperature was the key factor regulating soil respiration in the control and N addition treatments, which resulted in an apparent seasonal variation of soil respiration. The twofactor model, involving soil temperature and moisture, well fitted the variation of soil respiration in response to the temperature and moisture in the three temperate forests, and explained the 47%-87% variation of soil respiration. Furthermore, N addition regulated to some extent the response of soil respiration to soil temperature and moisture, and soil respiration increased faster and was more sensitive under conditions of relatively higher temperature and lower moisture under N addition. These findings revealed the shortterm effects of N addition on soil respiration in different temperate forests in North China, however, its underlying mechanisms is still unclear.
    Effects of different stoverincorporation ways on CO2 emission in dryland brown soil and soil carbon pool management index.
    YANG Xu, LAN Yu, MENG Jun, CHEN Wen-fu**
    2015, 34(3):  805-809. 
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    A field experiment was conducted to investigate the effects of corn stover returning and biochar application on the CO2 emission and carbon pool management index (CPMI) in dryland brown soil. The results showed that both stover returning and biochar application increased the cumulative CO2 emission of soil significantly by 132% and 76%, respectively, compared with the control. Under the same level of fertilization, biochar application significantly decreased the cumulative emission of CO2 by 24% compared with stover returning. Biochar played a positive role in CO2 emission reduction, in contrast with stover direct returning. Stover returning significantly increased CPMI, and this effect of stover direct returning was more significant compared with biochar application.
    Spatial variability of heavy metal contents and contamination assessment in forest soils of Daxing’an Mountains.
    ZHANG Ji-zhou1, LU Pin1,2**, WANG Li-min1, YU Zhi-min1
    2015, 34(3):  810-819. 
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    Soil heavy metal pollution is a potential hazardous factor to forest health. However, few studies have conducted on heavy metal contents, spatial variability and pollution status of natural forest soils in Daxing’an Mountains. In the study, the contents of Cd, Cr, Pb, Cu, Zn, Ni, Hg and As in forest soils of Daxing’an Mountains were analyzed. The spatial variability of these heavy metal elements mentioned above and their pollution potential in the region were evaluated by using GIS spatial analysis combined with classic heavy metal pollution assessment methods. The results showed that: (1) The spatial distributions of heavy metal contents exhibited moderate spatial heterogeneity, with variation coefficients from 16.67% to 35.39%. C0/(C0+C) values of Cd, Cr, Pb, Zn, Hg and As were less than 25%, suggesting that the spatial variability of their contents was mainly determined by structural factors, such as soil parent material and topography. C0/(C0+C) values of Cu and Ni varied in the range of 25%-75%, which means that these metals were mainly affected by non-structural factors and human activities.  The distribution patterns of heavy metal contents in the region presented as patchy, banded and island modes. (2) The contents of the eight heavy metals in the region showed a trend of accumulation. However, the average and maximum contents of the eight heavy metals were below the soil threshold levels of the Second Grade Criteria of the China’s Environmental Quality Standard (GB 15618-1995). The maximum contents of Cu, Zn and As exceeded the soil threshold levels of the First Grade Criteria, and the maximum contents of the other five elements were below the soil threshold levels of the First Grade Criteria. (3) As showed by the Geo-accumulation index, there was no pollution risk of Cr, Pb and Ni in the region, while the pollution risk of the other five elements was low. The Nemerow pollution index showed that slight pollution of heavy metals existed for overall soil quality in the region. Single factor ecological risk index showed that no potential risk was detected for Cu, Ni, Pb, Cr, Zn and As contents; however, the contents of Hg and Cd from some sites showed pollution risk. The content of Hg from some sites even reached the Third Pollution Level (moderate risk). The total potential ecological risk index  of 80% samples in the study area was at the riskfree level, and that of 20% of the samples was at the general level of risk.
    Effects of endophytic fungus (Trichoderma brevicompactum) on the decomposition of pruned tea leaves and soil fungi
    HU Yun-fei1,2, LI Rong-lin1, YANG Yi-yang1, ZHANG Yue2, LI Xing-hui2**
    2015, 34(3):  820-825. 
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    In order to study the degradation of pruned tea leaves by Trichoderma brevicompactum, a pot experiment was designed, which included four treatments: CK (pruned tea leaves and sterilized water), BY (pruned tea leaves and sterilized fermentation broth of T. brevicompactum), FG (pruned tea leaves, T. brevicompactum and sterilized water), and (pruned tea leaves, T. brevicompactum and sterilized fermentation broth). The changes in soil fungi were detected by PCRDGGE. The results showed that the application of T. Brevicompactum not only accelerated the softening speed of pruned tea leaves but also improved the browning degree of pruned tea leaves. After being treated for 60 days, the degradation rate of cellulose in pruned tea leaves increased by 18.16% in FG treatment and 19.02% in BF treatment, compared with CK and BF respectively. An obvious promotion effect on lignin degradation was observed in FG and BF treatments, and the degradation rate of lignin was accelerated during 30-45 days. The degradation rates of lignin in FG and BF treatments were increased by 12.49% and 9.61% after 60 days of incubation, compared with CK and BF respectively. At the early stage, cellulase and lignin enzyme activities were much higher in FG and BF than in Ck and BY, respectively, while they were insignificantly lower than that in CK at the later stage. After 60 days, there were some differences in the quantity of soil fungal communities between the treatment groups, but their community structure was similar. In conclusion, T. brevicompactum can improve the decomposition of pruned tea leaves and has little effect on soil fungal communities.
    Effects of different fertilization regimes on community structure and abundance of soil microorganisms in greenhouse vegetable rhizosphere soils.
    WANG Ya-nan1, ZENG Xi-bai1**, WANG Yu-zhong2, BAI Ling-yu1, LI Lian-fang1, WU Cui-xia1, E Sheng-zhe3, MA Shi-ming1
    2015, 34(3):  826-834. 
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    The effects of five fertilization regimes including CK, MNPK, 1/2MNPK, M and NPK on the community structure and abundance of rhizosphere bacteria, ammonia oxidizing bacteria and nirKtype denitrifying bacteria were investigated by the combination of terminal restriction fragment length polymorphism (T-RFLP) and realtime quantitative PCR in longterm greenhouse vegetable soils in Wuwei City, Gansu Province. The results showed that the community structure and abundance of bacteria, ammonia oxidizing bacteria and nirKtype denitrifying bacteria were significantly influenced by both fertilization regimes and rhizosphere effects. Fertilization regimes changed the main populations of the rhizosphere bacteria, ammonia oxidizing bacteria and nirKtype denitrifying bacteria, but their influences were discrepant in different functional microorganisms. The highest abundances of 16S rRNA gene, amoA gene and nirK gene were detected in M, MNPK and M treatments, which were 6.60×109, 1.85×108 and 3.49×107 copy numbers per gram dry soil, and 2.74, 2.81 and 3.46 times of that in CK treatment, respectively. Soil pH, soil organic matter and total phosphorous contents were the key factors influencing the abundances of bacteria, ammonia oxidizing bacteria and nirKtype denitrifying bacteria in greenhouse vegetable rhizosphere soils.
    Quick implementing of generalized additive models using R and its application in blue-green algal bloom forecasting.
    DENG Jian-ming1,2, QIN Bo-qiang1**, WANG Bo-wen3
    2015, 34(3):  835-842. 
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    Generalized additive models (GAM) is applicable to analyze nonlinear and nonmonotonic relationships between the response variable and explanatory variables. Increased attention has been paid to GAM in ecology study recently. In this study, longterm monitoring data from Lake Taihu were used to analyze the effect of environmental factors on Microcystis biomass. Data screening, link function confirmation, model selection and assessment were carried out by R. The results indicated that water temperature, total phosphorus, chemical oxygen demand and electrical conductivity were the main environmental factors that affected Microcystis biomass in Lake Taihu. The use of selfcoding functions can be effective in reducing code numbers. In addition, R+RStudio might be the optimal programming environment.
    Using GIS and MaxEnt to analyze the potential distribution of Abies chensiensis.
    GAO Bei1,3, WEI Hai-yan1**, GUO Yan-long1, GU Wei2**
    2015, 34(3):  843-852. 
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    Abies chensiensis is listed as a national thirdclass endangered wildlife species. It is one of the evergreen coniferous trees that are distributed primarily in Qinling Mountain across a range of elevations extending from 1350 m to 2500 m. Using the current geographic distribution records of A. chensiensis in Qinling Mountains and 20 factors, including 14 climate factors, 3 soil factors and 3 topographic factors, the potential geographic distribution of A. chensiensis in Qinling Mountains was assessed by MaxEnt model and ArcGIS spatial analysis. Results showed that the major factors impacting the suitable distribution area of A. chensiensis included 6 climate factors (annual mean temperature, annual extreme highest temperature, average temperature in January, ≥0 ℃ accumulated temperature, aridity index and annual sunshine duration), 1 soil factor (soil pH) and 1 terrain factor (elevation). The most suitable and suitable areas of A. chensiensis in Qinling Mountains were 19498.87 and 32219.61 km2, respectively, and were mainly concentrated in southeastern Gansu, central and southern Shaanxi, northeastern Sichuan, northwestern Hubei and northwestern Henan. With complex terrain, there were lots of secondary vegetations in these areas. The marginally suitable and unsuitable areas of A. chensiensis were 51874.76 and 106307.97 km2, respectively. This study showed that the maximum entropy model and ArcGIS spatial analysis could be used to regionalize the potential geographic distribution of A. chensiensis, providing information for the resource conservation and management of A. chensiensis.
    Carbon sequestration and carbon emission in eco-economic systems in Yinchuan City.
    PEI Yin-bao, LIU Xiao-peng**, LI Yong-hong
    2015, 34(3):  853-859. 
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    On the basis of the theory of ecoeconomics, statistics and geography, material flow analysis (MFA) model and exponential smoothing (ES) were used in this study to figure out the situations of carbon sequestrations and carbon emissions. Furthermore, the carbon flow in the ecoeconomic systems in Yinchuan from 2006 to 2012 was analyzed. Results showed that: (1) The total amount of carbon sequestration in ecosystems showed a trend of fluctuant growth, while the amount of carbon emission in economic systems kept rising, being far higher than the former. The detained amount of carbon in ecoeconomic systems was expanded excessively. (2) Farmland and wetland in the ecosystems of Yinchuan were the main carbon reservoir. The proportions of carbon sequestration in maize, rice and wheat were greater City  artificial wetland, lake and swamp mainly stored carbon. However, the space of carbon sink was squeezed by the nongreen space, which made the amount of carbon sequestration grow slowly. Moreover, energy, transportation and building were the three main carbon sources in Yinchuan City. Energy carbon emission concentrated on the consumptions of raw coal, crude oil and natural gas. In addition, the amount of carbon emission in highway freights and underconstructions grew rapidly in the period studied. (3) In the next five years, the amount of carbon sequestration in the city would be increased, with a growth rate of 3.96% annually. The increment of carbon emission would be up to 1140.92×104 t annually, which was predicted to decrease. Thus, the carbon emission would be in a phase of high benchmark and low growth rate. The results mentioned above suggest that ecological cost for economic development in Yinchuan City had been too expensive and economic development depended more on the ecological environment. Therefore, a low carbon transition during the development is imperative in the future.
    Ecological sensitivity evaluation of shorelines in Zhuhai, Guangdong Province.
    LIU Xiao-nan, CHENG Jiong, LI Cheng**
    2015, 34(3):  860-869. 
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    Shorelines play important roles in protecting aquatic ecosystem. Due to inappropriate and excessive utilization, erosion and damage have been found in parts of shorelines. Thus, how to rationally exploit and use shorelines has become an increasing concern. In this study, the city of Zhuhai was taken as an example to conduct an ecological sensitivity evaluation. A 500 m buffer zone along shorelines in Zhuhai was made and within this buffer zone, factors such as slope, the normalized difference vegetation index, the importance of biodiversity protection, and distance from shoreline to the basic farmland and fragile ecosystem were selected and single factor and multifactor ecological sensitivity evaluations were conducted. We then assigned the maximum sensitivity level observed within the buffer zone to the shorelines. In the meantime, the overlapping and ordering method was developed to identify the key affecting factors in a given sensitive area. Accordingly, recommendations of development and utilization of shorelines were proposed. Results showed that: (1) The single factor and multifactor ecological sensitivity evaluations showed the similar spatial pattern, which was generally at low sensitivity (nonsensitive or lightsensitive) in the study area, with a relatively few moderate and highsensitive areas clumped in the districts of Doumen and Xiangzhou; (2) For the ecological sensitivity evaluation of single factors, the area percentage for each ecological sensitivity level varied with factor level. The largest area percentages of high sensitivity level occurred under these factor conditions: slope between 25-45 degree, normalized differences vegetation index higher than 0.45, moderate biodiversity protection importance, distance to the basic farmland within 300-500 m, and distance to the fragile ecosystem less than 100 m; (3) The key affecting factors in lightly, moderately and highly sensitive areas were the distance to basic farmland, the distance to basic farmland along with the importance of biodiversity protection, and the distance to fragile ecosystem along with the importance of biodiversity protection; (4) Different development and utilization measures should be taken for shorelines of different sensitive levels, i.e., strictly controlling development, restricted development, and harmonious protection in the high sensitivity areas, moderate sensitivity areas, and light/nonsensitivity areas, respectively.
    Some research progresses in influencing factors for the efficiency of contaminated soil phytoremediation.
    MIAO Xin-yu, ZHOU Qi-xing**
    2015, 34(3):  870-877. 
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    Based on recent results of phytoremediation techniques applied in curbing environmental contamination, various factors influencing the efficiency of phytoremediation of contaminated soils were analyzed and summarized. First of all, three aspects of the main factors of phytoremediation efficiency of heavy metal pollutants and organic pollutants in soil were analyzed. That is, the physical and chemical properties of pollutants and their interaction effects, soil and climate conditions, and plant species, rhizosphere effects and cultivation measures. And then, the influence of additives on the phytoremediation efficiency was elaborated. Finally, issues for further research on phytoremediation of contaminated soil were discussed, including: providing good environment to promote plant growth and development, improving and strengthening the phytoremediation according to the factors that influence the efficiency of phytoremediation, and applying plant additives reasonably to improve the efficiency of phytoremediation.

     
    Research progress of persistence and horizontal gene transfer of recombinant DNA from genetically modified plants in soil environment.
    WU Yuan-feng1, LI Gang1, JI Guo-zhen1,2, SONG Xiao-long1, ZHAO Jian-ning1, YANG Dian-lin1, XIU Wei-ming1,2**
    2015, 34(3):  878-884. 
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    Horizontal gene transfer (HGT) is an important part of the environmental risk assessment of genetically modified plants. Exogenous recombinant DNA can be released from transgenic plants into the soil environment through root exudates, pollen, debris, etc. Studies have shown that the recombinant DNA could be integrated into the genome of soil microorganisms by homologous recombination. This would have direct or indirect effects on the structure and function of soil microbial community, and even result in the changes of soil ecosystem. This article focused on the persistence and HGT of the recombinant DNA in soil environment and the influence factors and detection methods, discussed the research highlights of the persistence and HGT of recombinant DNA, compared the research methods, and proposed the focus of future research in order to provide technical support for the risk assessment and security management of genetically modified plants.
    The progress and prospect on consecutive monoculture problems of Panax notoginseng.
    SUN Xue-ting1,2, LI Lei1,2, LONG Guang-qiang2, ZHANG Guang-hui2, MENG Zhen-gui2, YANG Sheng-chao2, CHEN Jun-wen1,2**
    2015, 34(3):  885-893. 
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    Panax notoginseng is a rare traditional Chinese medicinal material, but its cultivation has been seriously affected by consecutive monoculture problems (CMP). The possible reasons for CMP include continuous croppingdriven changes in microbial community structure and soilborne diseases, in allelochemical accumulation and autotoxic effect, and in soil physical and chemical properties and so on. Previous efforts were focused on the effect of a single factor on CMP of P. notoginseng or on its direct prevention and remedy of pest and disease in replanted soil. However, few attentions were paid to the internal interconnections and interactions among the various factors inducing the CMP. In this review, recent progresses on the mechanism of CMP in P. notoginseng were presented. The possible relationships among various factors were discussed, and an evaluation method for effects of various factors on the index of CMP was proposed. The future study on the CMP of P. notoginseng was also suggested. This review would provide a better understanding of the CMP, and also a theoretical basis for mitigating the CMP of this herb.
    Research progress on soil infiltration of green space and its importance for municipal ecological security.
    WU Hai-bing, FANG Hai-lan**
    2015, 34(3):  894-900. 
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    Soil infiltration is the process of water penetrating into soils through surface to form soil water, and it is one of the most important indexes to evaluate the capacity of adjusting soil water. This paper introduces the basic concepts, experimental methods and the major influencing factors on soil infiltration. The important functions of soil infiltration in rainflood reduction, groundwater supply, pollutant purification and plant growth are discussed in detail. The situation and influencing factors of soil infiltration in Chinese urban green space are also analyzed. Based on the importance of soil infiltration and insufficient consciousness of most people in China, we put forward four lines of suggestion on the research and application of soil infiltration as follows: (1) Soil infiltration should be taken as an index to evaluate the soil quality of urban green space in China as early as possible, and to improve the soil quality evaluation system. (2) Basic research on soil infiltration of green space should be strengthened. (3) Application of the techniques to improve soil infiltration should be enhanced and the infiltrationimproved integration technology that is suitable for application in China should be developed. (4) Soil construction and maintenance of the greening projects should be changed.