Cumulative effect of solar ultraviolet B radiation on pollen germination and tube growth of19 species in vitro

Abstract

Abstract: In order to determine the response of pollen to UV-B irradiation and the cumulative effects of UV-B exposure time on pollen germination and tube growth of plants,19 species were investigated in vitro by exposing their pollen grains to two levels of enhanced ultravioet B(UV-B_BE)(280～320 nm,350 and 500 mW/m² biologically effective UV-B radiation), simulating8% and 21% stratospheric ozone depletion in Lanzhou, China (36.04°N,1550 m),and to no UV-B (control group).Compared with the control,enhanced UV-B radiation significantly inhibited pollen germination and tube growth in most species.Higher UV-B flux rate caused greater inhibitory effect than lower UV-B radiation level.Several test plants exhibited insensitivity,even stimulation of pollen germination and tube growth to UV-B. The reduction in pollen germination rate and tube growth increased with exposure time, which indicated a cumulative effect of UV-B radiation.It is concluded that changes in pollen susceptibility to UV-B would have a serious ecological consequence under natural conditions.

CLC Number:

Q945

FENG Huyuan, AN Lizhe, TAN Lingling, HOU Zongdong, WANG Xunling . Cumulative effect of solar ultraviolet B radiation on pollen germination and tube growth of19 species in vitro[J]. Chinese Journal of Applied Ecology, 2002, (7): 814-818.

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