Abstract: The test of organophosphorus pesticide on toxicity of soil animal showed that soil animal is sensitive to organophophorus toxicity,the species and quantities of soil animal treated by four kinds of pesticide are decreased. The species of soil animals was decreased with decreasing common population and rare population,while the amount change of soil animal was associated with the amount change of dominant populations of Acarina and Collembola. Toxicology and toxicity experiment showed that the pesticide has a deadly effect to earthworm,and its toxicity constrained the respiration intensity of big soil animals obviously,which weakened with the increasing pesticide concentration and prolonging poisoning time.