Welcome to Chinese Journal of Ecology! Today is Share:

cje

Previous Articles     Next Articles

Effects of elevated O3 concentration and warming on oxidative jury and antioxidant enzyme activities in leaves of Cleome spinosa.

QIN Zi-qing1,2, XU Sheng2,3,4*, QI Shu-yan1, CHEN Wei2,3,4,5, HE Xing-yuan2,3,4,5, WANG Yi-jing2,4   

  1. (1Shenyang University, Shenyang 110044, China; 2Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 3Key Laboratory of Forest Ecology and Management, Chinese Academy of Sciences, Shenyang 110016, China; 4University of Chinese Academy of Sciences, Beijing 100049, China; 5Shengyang Arboretum, Chinese Academy of Sciences, Shenyang 110016, China).
  • Online:2020-03-10 Published:2020-03-10

Abstract: An experiment was conducted to examine the oxidation and anti-oxidizing response in leaves of Cleome spinosa, a garden ornamental plant, exposed to different ozone concentrations (80 and 160 nmol·mol-1) and increasing temperature treatments (2 ℃ higher than control) in open-top chambers (OTCs). The results showed that: (1) A visible leaf injury was observed after seven days with gas fumigation by elevated O3 (160 nmol·mol-1), and a large number of bleaching and chlorotic patches appeared on the leaves, whereas no visible injury was observed under 80 nmol·mol-1. (2) Under high O3 concentration (160 nmol·mol-1), malondialdehyde content (MDA), relative conductivity, peroxidase (POD) activity and superoxide dismutase (SOD) activity of C. spinosa leaves significantly increased by 175.7%, 259.9%, 111.4%,54.3%, respectively (P<0.05), while superoxide radical (O2-·) production rate was significantly reduced by 67% (P<0.05). Under the treatment of ozone concentration of 80 nmol·mol-1, MDA content increased significantly by 65.5% (P<0.05), catalase (CAT) activity decreased significantly by 65.9% (P<0.05), indicating that C.spinosa had high O3 tolerance ability at the concentration of 80 nmol·mol-1 O3. Under the O3 concentration of 160 nmol·mol-1 O3, plant leaves suffered O3 stress, and they resisted oxidative damage mainly by increasing antioxidant activity (POD and SOD). (3) Under single increasing temperature (IT), MDA content and relative conductivity of C. spinosa leaves increased significantly by 62.1% and 58.1% (P<0.05) respectively, but the O2-· production rate and CAT activity were 28.8% and 71.1% lower than the control respectively (both P<0.05), POD and SOD activities were not significantly different from the control, which may be attributed to enhanced plant metabolism due to higher temperature. (4) Compared with 80 nmol·mol-1 O3 alone, CAT, POD and SOD activities under the combination of increasing temperature and elevated O3 (80 nmol·mol-1) significantly increased by 141.7%, 72.1% and 107.9%, respectively (P<0.05); O2-· production rate significantly decreased by 51.0% (P<0.05), indicating that the combined treatment of O3 and increasing temperature exacerbated the O3 stress suffered by C. spinosa. These findings will provide a scientific basis for the selection and cultivation of plant species in gardens and urban greening in China under the sudden acute events of high-concentration ozone pollution.