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Effects of exogenous ATP on the growth of Brassica campestris seedlings under salt stress.

LAI Jing, LI Qiao-li, ZHANG Xiao-hua, LIANG Juan-hong, ZHANG Teng-guo*   

  1. (College of Life Sciences, Northwest Normal University, Lanzhou 730070, China).
  • Online:2020-06-10 Published:2020-06-10

Abstract: We examined the effects of exogenous ATP treatment on the growth of Brassica campestris seedlings under salt stress, and clarified the roles of signal molecules, hydrogen peroxide (H2O2) and calcium ion (Ca2+) in the ATP regulation of salt tolerance. The results showed that, compared with the single NaCl treatment, ATP+NaCl treatment decreased the number of dead cells, ROS (O2-· and H2O2) content, ion (Ca2+, Na+, Cl-) content, MDA content, Na+/K+ ratio, and relative electrical conductivity. The chlorophyll, proline, soluble sugar content, antioxidant enzyme (SOD, POD, CAT, APX) activity, transcriptional regulation of antioxidant enzyme genes (CAT, SOD, APX, GR), NADPH oxidase genes (RBOHD, RBOHF), P5CS1 gene, MAPK kinase genes (MAPK3, MAPK6), and salt tolerance genes (NHX1, SOS1) were all elevated. Compared with ATP+NaCl treatment, the relative electrical conductivity, MDA, chlorophyll, proline, soluble sugar, the activities of antioxidant enzymes (SOD, POD, CAT, APX), and the expression of the above genes were decreased when treated with ATP+NaCl+inhibitors (DPI, DMTU and EGTA). These results indicated that exogenous ATP application could increase cell activity, ROS content, ion content, chlorophyll content, osmotic regulator, antioxidant enzyme activity and related gene expression in B. campestris under salt stress. In addition, signal molecules of H2O2 and Ca2+ were involved in the ATP regulation of enhancing salt tolerance in B. campestris.

Key words: ecological suitable region, ecological characteristics., maximum entropy model, Codonopsis pilosula