五氯酚(PCP)污染土壤厌氧生物修复技术的初步研究

应用生态学报 ›› 2001, Vol. ›› Issue (3): 439-442.

五氯酚(PCP)污染土壤厌氧生物修复技术的初步研究

徐向阳, 冯孝善

浙江大学环境工程系, 杭州 310029

收稿日期:2001-02-16 修回日期:2001-03-20 出版日期:2001-05-25
通讯作者: 徐向阳,男,1964年生,博士,副教授.主要从事环境微生物与生物技术、废物生物处理等领域的研究,发表论文50篇.
基金资助:
国家自然科学基金资助项目(30070026)

Preliminary study on anaerobic bioremediation process for pentachlorophenol (PCP)-contaminated soil

XU Xiangyang, FENG Xiaoshan

Depart.Environ.Eng., Zhejiang University, Hangzhou 310029

Received:2001-02-16 Revised:2001-03-20 Online:2001-05-25

摘要/Abstract

摘要： 研究土壤泥浆反应器在投加厌氧颗粒污泥条件下修复PCP污染土壤的性能.结果表明,对PCP浓度30mg·kg^-1模拟污染土壤,其土壤泥浆中土著性厌氧微生物对PCP具有一定的还原脱氯降解活性,28d平均PCP降解速率为0.258mg·kg^-1·d^-1;投加5.0g.kg^-1颗粒污泥的土壤泥浆反应器在好氧、好氧-厌氧、厌氧操作条件下,28d平均PCP降解速率为0.269、0.291/0.842、0.892mg·kg^-1·d^-1.投加5、10、25g·Kg^-1颗粒污泥的土壤泥浆反应器在厌氧条件下,35d平均PCP降解速率分别达到0.834、0.843、0.928mg·kg^-1·d^-1,PCP降解速率随颗粒污泥投加量的增加而增大;对PCP浓度60mg·kg^-1模拟污染土壤,投加颗粒污泥的泥浆反应器仍可获得相对较高的初始PCP降解速率.

Abstract: The performances of soil slurry process with supplement of anaerobic granular sludge for remediating PCP contaminated soil was investigated.The results showed that inherent anaerobic microbes in soil exhibited a little dechlorinating activity for simulative contaminated soil with 30mg穔g^-1 of PCP,the average PCP degradation rates within 28 d was 0.258 mg穔g^-1 穌^-1.In soil slurry processes with 5 g穔g^-1 granular sludge at aerobic,aerobic /anaerobic and anaerobic operation,the average PCP degradation rates within 28 d were 0.269,0.291/0.842 and 0.892mg穔g^-1 穌^-1,respectively. PCP degradation rates were increased with increasing the amounts of anaerobic granular sludge,0.834, 0.843,0.928mg穔g^-1 穌^-1 of the average PCP degradation rates within 35 d were achieved in soil slurry processes supplemented with 5,10,25 g穔g^-1 of granular sludge,respectively.This bioremediation process also presented higher initial PCP degradation rate for higher concentration(60mg穔g^-1 ) of PCP in soil.

中图分类号:

X703.1

徐向阳, 冯孝善. 五氯酚(PCP)污染土壤厌氧生物修复技术的初步研究[J]. 应用生态学报, 2001, (3): 439-442.

XU Xiangyang, FENG Xiaoshan . Preliminary study on anaerobic bioremediation process for pentachlorophenol (PCP)-contaminated soil[J]. Chinese Journal of Applied Ecology, 2001, (3): 439-442.

参考文献

[1] Ahring BK, Christiansen N. 1994. Introduction of bioremediation ability into granular sludge. Wat Sci Tech nol, 29 (5～6):189～193 [2] Bhatnagar L, Fathepure BZ. 1991. Mixed culture in detoxification of hazardous waste. In Zeikus JG eds. Mixed Cultures in Biotechnology.New York:McGraw-Hill Book Co. 293～340 [3] Haggblom MM, Valo RJ. 1995. Bioremediation of chlorophenol waters. In:Young LY eds. Microbial Transformation and Degradation of Toxic Organic Chemicals. New York:Wiley-Liss. 389～434 [4] Hu W-J(胡望钧).1993. Emergency dispose techniques and monitoring methods for environmental accidents by commonly toxic chemicals.Beijing:China Environmental Science Press. 359～362(in Chinese) [5] Laine MM, Jorgensen KS. 1997. Effective and safe composting of chlorophenol-contaminated soil in pilot-scale. Environ Sci Technol, 31(2):371～378 [6] Leffler FE, Sanford RA, Tiedje JM. 1996. Initial characterization of a reductive dehalogenese from Desulfitobacterium chlororesprians C023. Appl Environ Microbiol, 62(10):3809～3813 [7] Mikesell MD, Boyd SA. 1988. Enhancement of PCP degradation in soil through induced anaerobisis and bioaugementstion with anaerobic sewage sludge. Environ Sci Technol, 22(12):1411～1414 [8] Natarajan MR, Wang H, Hickey R. 1995. Effect of oxygen and storage conditions on the metabolic activities of polychlorinated biphenyls dechlorinating microbial granules. Appl Microbial Biotechnol, 43:733～738 [9] Pagano JJ, Scrudato RJ, Roberts RN et al. 1995. Reductive dechlotination of PCB-contsiminated sediments in an anaerobic bioreactor system. Environ Sci Technol, 29(10):2584～2589 [10] Tiedje JM, Quensen JF, Mohn WW et al. 1991. Reductive dechlorination of chlorinated aromatic poputsnts. In:Rossmrre RW eds. Biodeterioration and Biodegradation. New York:Elsevier Applied Science. [11] Xu X-Y(徐向阳). 1997. Studies on characterization of anaerobic PCPdegrading granular sludge and its application in bioremediation of contaminated environment(Ph. D dissertation). Hangzhou:Zhejiang University. (in Chinese)