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应用生态学报 ›› 2002, Vol. ›› Issue (6): 701-704.

• 研究论文 • 上一篇    下一篇

球孢白僵菌对桃蚜接种后特定时间内的侵染率

许寿涛, 冯明光, 应盛华   

  1. 浙江大学微生物研究所, 杭州 310029
  • 收稿日期:2001-04-11 修回日期:2001-06-25 出版日期:2002-06-15
  • 通讯作者: 冯明光,E-mail:mgfeng@cls.zju.edu.cn
  • 基金资助:
    国家杰出青年科学基金项目(39525004);国家自然科学基金(39870513)资助项目

Time-specific infection rate of Beauveria bassiana on Myzus persicae after topical inoculation of conidial suspension

XU Shoutao, FENG Mingguang, YING Shenghua   

  1. Research Institute of Microbiology, Zhejiang University, Hangzhou 310029
  • Received:2001-04-11 Revised:2001-06-25 Online:2002-06-15

摘要: 用球孢白僵菌(Beauveria bassiana)BBSG8702菌株的分生孢子悬液(5×106个·ml-1孢子)对桃蚜(Myzus persicae)始产若蚜的成蚜进行表面接种,在接种后56h内,每隔8h取样用0.2%百菌清处理蚜虫,使其体表残存的孢子全部失活,分别置于10℃和20℃下逐日观察感染引起的死亡.结果表明,10℃下接种后56h、20℃下接种后40h内各时间段有效侵染引起的死亡率相互间存在显著差异,并与不用杀菌剂的对照处理差异显著(P<0.05).与对照相比,接种后8、16、24、32、40和56h内,10℃下的有效侵染率分别为22.9、48.8、64.9、80.4、72.7和98.3%,20℃下分别为31.6、48.8、58.6、86.9、97.2和98.7%.由此表明,在10~20℃范围内,接种后24h内是该菌有效侵染桃蚜的关键时段,有效侵染率达59~65%.

Abstract: Conidial suspension(5×106conidia·ml-1) of the entomopathogenic fungus,Beauveria bassiana BBSG8702 was used to inoculate newly emerged apterous adults of the green peach aphid (Myzus persicae) on detached cabbage leaves in order to measure time-specific infection rate of the fungal agent. The aphids of conidia attached were disinfected using0.2% chlorothalonil solution at intervals of 8~56 h after inoculation and then maintained at 10 and 20℃ for daily observation. There was a significant difference (P<0.05) in mortality attributed to the fungal infection among the time intervals within56 h at 10℃ after inoculation or within40 h at 20℃.Compared with the control (not treated with 0.2% chlorothalonil after inoculation),time-specific infection rate at 10℃ was estimated as 22.9%,48.8%,64.9%,80.4%,72.7%,and 98.3% within8 h,16 h,24 h,32 h,40 h,and 56 h after inoculation, respectively. The same estimates at 20℃ were 31.6%,48.8%,58.6%,86.9%, 97.2%,and 98.7%, respectively. The results indicate that the first 24 h after inoculation was crucial to the infection of B. bassiana to M. persicae. Effective infection with the first 24 h accumulated to 59~65% at 10~20℃.

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