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应用生态学报 ›› 2003, Vol. ›› Issue (4): 545-548.

• 研究论文 • 上一篇    下一篇

球孢白僵菌分生孢子乳悬剂对甘蓝上桃蚜的田间控制效果

应盛华1, 冯明光1, 许寿涛1, 马再波2   

  1. 1. 浙江大学微生物研究所, 杭州 310029;
    2. 云南星耀生物技术有限公司, 昆明 650051
  • 收稿日期:2001-10-26 修回日期:2002-03-26 出版日期:2003-04-15
  • 通讯作者: 冯明光
  • 基金资助:
    “长江学者奖励计划”;国家自然科学基金(39870513);云南省省院省校科技合作资助项目(2000YZ03).

Field efficacy of emulsifiable suspensions of Beauveria bassiana conidia for control of Myzus persicae population on cabbage

YING Shenghua1, FENG Mingguang1, XU Shoutao1, MA Zaibo 2   

  1. 1. Institute of Microbiology, Zhejiang University, Hangzhou 310029, China;
    2. Yunnan Xingyao Biotechnology Co., Ltd., Kunming 650051, China
  • Received:2001-10-26 Revised:2002-03-26 Online:2003-04-15

摘要: 利用液酵法生产球孢白僵菌(Beauveria bassiana)SG8702菌株的分生孢子粉,配制成含孢量为1010个·ml-1的孢子乳悬剂I,在此基础上按1%(W/V)的比例添加10%吡虫啉可湿性粉剂而得孢子乳悬剂Ⅱ.于2001年7月对两种孢子乳悬剂在云南昆明进行了自然条件下甘蓝上桃蚜(Myzus persicae)的田间小区(5.5m×4.8m)药效试验,各3个浓度(含孢量分别为107、106及105个·ml-1常规喷雾处理,重复设清水喷雾对照,重复3次,随机区组排列.在喷菌后连续28d的田间蚜虫密度定期抽样调查中,乳悬剂Ⅱ的1000倍稀释液喷雾有效抑制了蚜虫数量增长,喷后第7~28天防效始终在90%以上.乳悬剂I的相同稀释液喷雾对桃蚜的控制略逊于乳悬剂Ⅱ,但喷后第7天的相对防效也达到85%,此后维持防效70%以上达两周,第24天和28天才分别降至64.4%和52.6%.用含孢量106和105个·ml-1的菌液喷雾,乳悬剂I仍表现出明显的控蚜效果,而添加微量吡虫啉的乳悬剂II的控蚜效果总是优于同一浓度下的乳悬剂I.昆明地区夏季温和而多小雨的气候有利于孢子乳悬剂发挥作用.

Abstract: The conidial powder of Beauveria bassiana SG8702 produced using a diphasic fermentation technology was formulated into emulsifiable suspention I (ES-I, >1010 conidia穖l-1) and ES-II, which resulted from adding1% (W/V) of imidacloprid 10% WP to the ES-I. Three dilutions (107, 106, and 105 conidia穖l-1 of each were sprayed onto cabbage plants for control of the green peach aphid Myzus persicae population in field plots in Kunming, Yunnan during July 2001. Each dilution and a water spray as control were replicated three times. During a 28 d period of sampling at 3 or 4 d intervals, spray of the ES-IIat 107 conidia穖l-1 controlled the increase of the aphid population by >90% consistently from day 7 after spray. The efficacy for the ES-Iat the same concentration reached 85% on day 7 after spray, exceeded 70% in the following two weeks, but declined to 64.4% on day 24 and 52.6% on day 28. At the lower concentrations sprayed, both suspensions significantly suppressed the increase of M. persicae population, but the ES-II resulted in higher efficacy than the ES-I. Local summer weather with mild temperature and frequent rainy days was favorable to the use of the formulations for aphid control.

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